BMRB Entry 10059

Title:
Solution Structure of the Band 7 Domain of the mouse Flotillin 2 Protein
Deposition date:
2006-11-29
Original release date:
2008-08-13
Authors:
Miyamoto, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Miyamoto, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the Band 7 Domain of the mouse Flotillin 2 Protein"  .

Assembly members:

Assembly members:
Band 7 Domain, polymer, 143 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P030818-03

Data sets:
Data typeCount
13C chemical shifts607
15N chemical shifts148
1H chemical shifts999

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Flotillin 21

Entities:

Entity 1, Flotillin 2 143 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLNARGILE
2   SERLEUGLUILEMETTHRLEUGLNPROARG
3   CYSGLUASPVALGLUTHRALAGLUGLYVAL
4   ALALEUTHRVALTHRGLYVALALAGLNVAL
5   LYSILEMETTHRGLULYSGLULEULEUALA
6   VALALACYSGLUGLNPHELEUGLYLYSASN
7   VALGLNASPILELYSASNVALVALLEUGLN
8   THRLEUGLUGLYHISLEUARGSERILELEU
9   GLYTHRLEUTHRVALGLUGLNILETYRGLN
10   ASPARGASPGLNPHEALALYSLEUVALARG
11   GLUVALALAALAPROASPVALGLYARGMET
12   GLYILEGLUILELEUSERPHETHRILELYS
13   ASPVALTYRASPLYSVALASPTYRLEUSER
14   SERLEUGLYLYSTHRGLNTHRSERGLYPRO
15   SERSERGLY

Samples:

sample_1: Band 7 Domain, [U-13C; U-15N], 1.02 mM; PiNa 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.8996, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAB23392 BAC26837 BAE28942 BAE41879 BAI46697
EMBL CAG46583 CAG47061
GB AAA65729 AAA93127 AAC98727 AAD00120 AAH03683
REF NP_001030543 NP_001035493 NP_001257729 NP_001257730 NP_001271156
SP A6QLR4 Q14254 Q60634 Q9Z2S9
TPG DAA18988
AlphaFold A6QLR4 Q14254 Q60634 Q9Z2S9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks