BMRB Entry 7430

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PDB ID: 2k8v
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR7430
MolProbity Validation Chart

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Title:
Solution structure of Reduced ERp18
Deposition date:
2008-09-25
Original release date:
2009-04-22
Authors:
Rowe, Michelle; Alanen, Heli; Ruddock, Lloyd; Kelly, Geoff; Schmidt, Jurgen; Williamson, Richard; Howard, Mark
Citation:

Citation: Rowe, Michelle; Ruddock, Lloyd; Kelly, Geoff; Schmidt, Jurgen; Williamson, Richard; Howard, Mark. "Solution Structure and Dynamics of ERp18: a Small ER Resident Oxidoreductase"  Biochemistry 48, 4596-4606 (2009).
PubMed: 19361226

Assembly members:

Assembly members:
ERp18, polymer, 157 residues, 17771 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHIA128

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ERp18: MHHHHHHMSDGHNGLGKGFG DHIHWRTLEDGKKEAAASGL PLMVIIHKSWCGACKALKPK FAESTEISELSHNFVMVNLE DEEEPKDEDFSPDGGYIPRI LFLDPSGKVHPEIINENGNP SYKYFYVSAEQVVQGMKEAQ ERLTGDAFRKKHLEDEL

Data sets:
Data typeCount
13C chemical shifts287
15N chemical shifts153
1H chemical shifts161

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1ERp181

Entities:

Entity 1, ERp18 157 residues - 17771 Da.

Residues 1-8 represent a non-native affinity tag

1   METHISHISHISHISHISHISMETSERASP
2   GLYHISASNGLYLEUGLYLYSGLYPHEGLY
3   ASPHISILEHISTRPARGTHRLEUGLUASP
4   GLYLYSLYSGLUALAALAALASERGLYLEU
5   PROLEUMETVALILEILEHISLYSSERTRP
6   CYSGLYALACYSLYSALALEULYSPROLYS
7   PHEALAGLUSERTHRGLUILESERGLULEU
8   SERHISASNPHEVALMETVALASNLEUGLU
9   ASPGLUGLUGLUPROLYSASPGLUASPPHE
10   SERPROASPGLYGLYTYRILEPROARGILE
11   LEUPHELEUASPPROSERGLYLYSVALHIS
12   PROGLUILEILEASNGLUASNGLYASNPRO
13   SERTYRLYSTYRPHETYRVALSERALAGLU
14   GLNVALVALGLNGLYMETLYSGLUALAGLN
15   GLUARGLEUTHRGLYASPALAPHEARGLYS
16   LYSHISLEUGLUASPGLULEU

Related Database Links:

BMRB 15964 16962
PDB 2K8V
DBJ BAG52132
GB AAD20035 AAH01493 AAH08913 AAH08953 AAN34781
REF NP_001253090 NP_056997 XP_001110583 XP_002915769 XP_003364421
SP O95881
AlphaFold O95881

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