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BMRB Entry 7400 BMRB - Biological Magnetic Resonance Bank

BMRB Entry 7400

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR7400
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution structure of rhodostomin P48A mutant
Deposition date:
2007-06-29
Original release date:
2009-10-12
Authors:
Chuang, W.; Liu, Y.; Shiu, J.
Citation:

Citation: Chuang, W.; Chen, C.; Shiu, J.; Liu, Y.; Hsieh, Y.; Chang, Y.; Chen, Y.; Lo, S.. "Dynamic Properties of the RGD Motif of Disintegrin Modulate its Recognition to Integrin a5b1"  .

Assembly members:

Assembly members:
Rhodostoxin-disintegrin_rhodostomin, polymer, 68 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Malayan pit viper   Taxonomy ID: 8717   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Calloselasma rhodostoma

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rhodostoxin-disintegrin_rhodostomin: GKECDCSSPENPCCDAATCK LRPGAQCGEGLCCEQCKFSR AGKICRIARGDMPDDRCTGQ SADCPRYH

Data sets:
Data typeCount
15N chemical shifts67
1H chemical shifts372

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rhodostoxin-disintegrin_rhodostomin1

Entities:

Entity 1, Rhodostoxin-disintegrin_rhodostomin 68 residues - Formula weight is not available

1   GLYLYSGLUCYSASPCYSSERSERPROGLU
2   ASNPROCYSCYSASPALAALATHRCYSLYS
3   LEUARGPROGLYALAGLNCYSGLYGLUGLY
4   LEUCYSCYSGLUGLNCYSLYSPHESERARG
5   ALAGLYLYSILECYSARGILEALAARGGLY
6   ASPMETPROASPASPARGCYSTHRGLYGLN
7   SERALAASPCYSPROARGTYRHIS

Samples:

sample_1: Rhodostoxin-disintegrin_rhodostomin 2 mM; H2O 90%; D2O 10%

sample_2: Rhodostoxin-disintegrin_rhodostomin 2 mM; D2O 100%

sample_3: Rhodostoxin-disintegrin_rhodostomin, [U-15N], 2 mM; H2O 90%; D2O 10%

sample_4: Rhodostoxin-disintegrin_rhodostomin, [U-15N], 2 mM; D2O 100%

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availableisotropicsample_conditions_1
2D TOCSYnot availableisotropicsample_conditions_1
2D NOESYnot availableisotropicsample_conditions_1
2D TOCSYnot availableisotropicsample_conditions_1
3D_15N-separated_NOESYnot availableisotropicsample_conditions_1
3D_15N-separated_TOCSYnot availableisotropicsample_conditions_1
HNHAnot availableisotropicsample_conditions_1
2D HSQCnot availableisotropicsample_conditions_1

Software:

xwinnmr v2.6, Bruker - collection

AURELIA v3.1.7, Bruker - data analysis

X-PLOR v3.185, Brunger - refinement

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

BMRB 17963 19210 19211 19212 2856 5117 5949 7399
PDB
GB AAA49196 AAA57568
SP P30403

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks