BMRB Entry 7377

Name: Resonance assignments of the metal sensor CzrA in the apo-, Zn2- and DNA-bound (42 kDa) states
Published: 2007-08-23

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7377

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Resonance assignments of the metal sensor CzrA in the apo-, Zn2- and DNA-bound (42 kDa) states

Deposition date:

2007-03-16

Original release date:

2007-08-23

Authors:

Arunkumar, Alphonse; Pennella, Mario; Kong, Xiangming; David, Giedroc

Citation:

Citation: Arunkumar, Alphonse; Pennella, Mario; Kong, Xiangming; Giedroc, David. "Resonance assignments of the metal sensor CzrA in the apo-, Zn2- and DNA-bound (42 kDa) states" Biomol. NMR Assignments 1, 99-101 (2007).
PubMed: 19636838

Assembly members:

Assembly members:
CzrA_Chain, polymer, 106 residues, 11988.69 Da.
DNA, polymer, 28 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Staphylococcus aureus Taxonomy ID: 1280 Superkingdom: Bacteria Kingdom: not available Genus/species: Staphylococcus aureus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 3d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CzrA_Chain: MAEQYSEINTDTLERVTEIF KALGDYNRIRIMELLSVSEA SVGHISHQLNLSQSNVSHQL KLLKSVHLVKAKRQGQSMIY SLDDIHVATMLKQAIHHANH PKESGL
DNA: TAACATATGAACATATGTTC ATATGTTA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	360
15N chemical shifts	114
1H chemical shifts	271

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	czra1	1
2	czra2	1
3	DNA1	2

Entities:

Entity 1, czra1 106 residues - 11988.69 Da.

1

MET

ALA

GLU

GLN

TYR

SER

GLU

ILE

ASN

THR

2

ASP

THR

LEU

GLU

ARG

VAL

THR

GLU

ILE

PHE

3

LYS

ALA

LEU

GLY

ASP

TYR

ASN

ARG

ILE

ARG

4

ILE

MET

GLU

LEU

SER

VAL

SER

GLU

ALA

5

SER

VAL

GLY

HIS

ILE

SER

HIS

GLN

LEU

ASN

6

LEU

SER

GLN

SER

ASN

VAL

SER

HIS

GLN

LEU

7

LYS

LEU

LYS

SER

VAL

HIS

LEU

VAL

LYS

8

ALA

LYS

ARG

GLN

GLY

GLN

SER

MET

ILE

TYR

9

SER

LEU

ASP

ILE

HIS

VAL

ALA

THR

MET

10

LEU

LYS

GLN

ALA

ILE

HIS

ALA

ASN

HIS

11

PRO

LYS

GLU

SER

GLY

LEU

Entity 2, DNA1 28 residues - Formula weight is not available

1

DT

DA

DC

DA

DT

DA

DT

DG

DA

2

DA

DC

DA

DT

DA

DT

DG

DT

DC

3

DA

DT

DA

DT

DG

DT

DA

Samples:

DNACzrA_sample_1: CzrA, [U-100% 13C; U-100% 15N; 80% 2H], 1 ± 0.1 mM; DNA1 – 1.5 mM; MES, [U-100% 2H], 10 ± 0.5 mM; sodium chloride 50 ± 1 mM

DNACzrA_sample_2: CzrA, [U-10% 13C], 1 ± 0.1 mM; DNA1 – 1.5 mM; MES, [U-100% 2H], 10 ± 0.5 mM; sodium chloride 50 ± 1 mM

DNACzrA_sample_3: CzrA, [U-2H; U-15N]; [13C]-Ile,Leu,Val-methyl, 1 ± 0.1 mM; DNA1 – 1.5 mM; MES, [U-100% 2H], 10 ± 0.5 mM; sodium chloride 50 ± 1 mM

DNACzrA_sample_4: CzrA, [U-2H; U-15N]; [13C]-Ile,Leu,Val-methyl; [U-2H]-Leu,Val-methyl, 1 ± 0.1 mM; DNA1 – 1.5 mM; MES, [U-100% 2H], 10 ± 0.5 mM; sodium chloride 50 ± 1 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	DNACzrA_sample_1	isotropic	sample_conditions_1
2D 1H-13C HMQC	DNACzrA_sample_1	isotropic	sample_conditions_1
3D TROSY-HN(COCA)CB	DNACzrA_sample_1	isotropic	sample_conditions_1
3D TROSY-HNCACB	DNACzrA_sample_1	isotropic	sample_conditions_1
3D TROSY-HNCA	DNACzrA_sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY-TROSY	DNACzrA_sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	DNACzrA_sample_1	isotropic	sample_conditions_1
4D 13C/15 NOESY	DNACzrA_sample_1	isotropic	sample_conditions_1
3D TROSY-HNCO	DNACzrA_sample_1	isotropic	sample_conditions_1
3D TROSY-HNCO (1J HN couplings)	DNACzrA_sample_1	anisotropic	sample_conditions_1
3D TROSY-HNCO (1J COCA couplings)	DNACzrA_sample_1	anisotropic	sample_conditions_1
3D TROSY-HNCO (1J NCA couplings)	DNACzrA_sample_1	anisotropic	sample_conditions_1
2D IPAP-HSQC	DNACzrA_sample_1	anisotropic	sample_conditions_1
3D HMCM[CG]CBCA	DNACzrA_sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

MARS, (MARS)-Jung and Zweckstetter - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz
Varian INOVA 800 MHz
Varian INOVA 900 MHz

BMRB	15177 7376
PDB	1R1U 1R1V 2KJB 2KJC 2M30 4GGG
DBJ	BAA36687 BAB43231 BAB58307 BAB95934 BAF68321
EMBL	CAG41214 CAG43856 CAI81718 CAQ50573 CBI50151
GB	AAC32484 AAW38447 ABD21735 ABD31419 ABQ49963
REF	NP_372669 NP_375252 NP_646886 WP_000003755 WP_000003756