BMRB Entry 7359

Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR7359

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Title:
1H, 15N and 13C chemical shift assignments for reduced and oxidised forms of the DsbA oxidoreductase from Vibrio cholerae
Deposition date:
2007-01-10
Original release date:
2007-06-26
Authors:
Horne, Henry; Scanlon, Martin
Citation:

Citation: Horne, James; Scanlon, Martin. "Backbone and side chain 1H, 15N and 13C assignments for the reduced form of the oxidoreductase protein DsbA from Vibrio cholerae"  Biomol. NMR Assignments 1, 75-76 (2007).
PubMed: 19636831

Assembly members:

Assembly members:
DsbA protein disulfide isomerase, polymer, 181 residues, 20443.3 Da.

Natural source:

Natural source:   Common Name: cholera   Taxonomy ID: 666   Superkingdom: Eukaryota   Kingdom: Not applicable   Genus/species: Vibrio cholerae

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pKc-type

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DsbA protein disulfide isomerase: AQFKEGEHYQVLKTPASSSP VVSEFFSFYCPHCNTFEPII AQLKQQLPEGAKFQKNHVSF MGGNMGQAMSKAYATMIALE VEDKMVPVMFNRIHTLRKPP KDEQELRQIFLDEGIDAAKF DAAYNGFAVDSMVRRFDKQF QDSGLTGVPAVVVNNRYLVQ GQSAKSLDEYFDLVNYLLTL K

Data sets:
Data typeCount
13C chemical shifts526
15N chemical shifts182
1H chemical shifts1157

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1DsbA1

Entities:

Entity 1, DsbA 181 residues - 20443.3 Da.

V148A cloning artefact in original clone gifted to this laboratory

1   ALAGLNPHELYSGLUGLYGLUHISTYRGLN
2   VALLEULYSTHRPROALASERSERSERPRO
3   VALVALSERGLUPHEPHESERPHETYRCYS
4   PROHISCYSASNTHRPHEGLUPROILEILE
5   ALAGLNLEULYSGLNGLNLEUPROGLUGLY
6   ALALYSPHEGLNLYSASNHISVALSERPHE
7   METGLYGLYASNMETGLYGLNALAMETSER
8   LYSALATYRALATHRMETILEALALEUGLU
9   VALGLUASPLYSMETVALPROVALMETPHE
10   ASNARGILEHISTHRLEUARGLYSPROPRO
11   LYSASPGLUGLNGLULEUARGGLNILEPHE
12   LEUASPGLUGLYILEASPALAALALYSPHE
13   ASPALAALATYRASNGLYPHEALAVALASP
14   SERMETVALARGARGPHEASPLYSGLNPHE
15   GLNASPSERGLYLEUTHRGLYVALPROALA
16   VALVALVALASNASNARGTYRLEUVALGLN
17   GLYGLNSERALALYSSERLEUASPGLUTYR
18   PHEASPLEUVALASNTYRLEULEUTHRLEU
19   LYS

Related Database Links:

BMRB 7360
PDB 1BED 2IJY 4DVC
DBJ BAP04237
EMBL CAA45977
GB AAA27568 AAF93212 ABQ20372 ACP04371 ACP08173
REF NP_229693 WP_000732279 WP_000732281 WP_000732282 WP_000732283
SP P32557
AlphaFold P32557

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks