BMRB Entry 7311

Name: Backbone 1H, 13C, and 15N and CB Assignments for Rat Islet Amyloid Polypeptide
Published: 2007-05-04

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7311

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N and CB Assignments for Rat Islet Amyloid Polypeptide

Deposition date:

2006-10-01

Original release date:

2007-05-04

Authors:

Williamson, Jessica; Miranker, Andrew

Citation:

Citation: Williamson, Jessica; Miranker, Andrew. "Direct Detection of Transient alpha-Helical States of Islet Amyloid Polypeptide" Protein Sci. 16, 110-117 (2007).
PubMed: 17123962

Assembly members:

Assembly members:
Rat Islet Amyliod Polypeptide, polymer, 38 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rat Islet Amyliod Polypeptide: KCNTATCATQRLANFLVRSS NNLGPVLPPTNVGSNTYX

Data sets:

assigned_chemical_shifts

Data type	Count
13C chemical shifts	111
15N chemical shifts	37
1H chemical shifts	73

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Rat Islet Amyloid Polypeptide	1

Entities:

Entity 1, Rat Islet Amyloid Polypeptide 38 residues - Formula weight is not available

1

LYS

CYS

ASN

THR

ALA

THR

CYS

ALA

THR

GLN

2

ARG

LEU

ALA

ASN

PHE

LEU

VAL

ARG

SER

3

ASN

LEU

GLY

PRO

VAL

LEU

PRO

THR

4

ASN

VAL

GLY

SER

ASN

THR

TYR

NH2

Samples:

sample_1: Rat Islet Amyliod Polypeptide, [U-13C; U-15N], 1.5 mM; potassium chloride 0.1 mM; potassium phosphate 0.05 mM; deuterium oxide 10%

conditions_1: pH: 5.5; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
CBCANH	sample_1	not available	conditions_1
CBCA(CO)NNH	sample_1	not available	conditions_1
HNCO	sample_1	not available	conditions_1
NOESY-15N-HSQC	sample_1	not available	conditions_1
TOCSY-15N-HSQC	sample_1	not available	conditions_1
15N-HSQC	sample_1	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Varian Unity-Inova 500 MHz

BMRB	16314 20046
PDB	2KJ7
DBJ	BAA22051
EMBL	CAA37003
GB	AAA37874 AAA40730 AAA41359 AAH27527 EDL10633
REF	NP_034621 NP_036718 XP_006155144 XP_006155145 XP_006237676
SP	P12968 P12969
AlphaFold	P12968 P12969