BMRB Entry 7288

Name: 1H, 13C, 15N Chemical Shift Assignments and 15N Relaxation Data for Transmembrane Domain of BNIP3
Published: 2007-05-10

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PDB ID: 2j5d
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7288
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, 15N Chemical Shift Assignments and 15N Relaxation Data for Transmembrane Domain of BNIP3

Deposition date:

2006-09-04

Original release date:

2007-05-10

Authors:

Pustovalova, Julia; Bocharov, Eduard; Maslennikov, Innokenty; Ermolyuk, Yaroslav; Goncharuk, Marina; Arseniev, Alexander

Citation:

Citation: Bocharov, Eduard; Pustovalova, Yulia; Pavlov, Konstantin; Volynsky, Pavel; Goncharuk, Marina; Ermolyuk, Yaroslav; Karpunin, Dmitry; Schulga, Alexey; Kirpichnikov, Michail; Efremov, Roman; Maslennikov, Innokenty; Arseniev, Alexander. "Unique dimeric structure of BNip3 transmembrane domain suggests membrane permeabilization as a cell death trigger." J. Biol. Chem. 282, 16256-16266 (2007).
PubMed: 17412696

Assembly members:

Assembly members:
BNIP3 TM, polymer, 45 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
BNIP3 TM: RNTSVMKKGGIFSAEFLKVF LPSLLLSHLLAIGLGIYIGR RLTTS

Data sets:

assigned_chemical_shifts
- chem_shift_list_1
heteronucl_NOEs
- heteronucl_NOE_list_1
heteronucl_T1_relaxation
- T1_list_1
heteronucl_T2_relaxation
- T2_list_1
H_exch_rates
- H_exch_list_1

Data type	Count
13C chemical shifts	114
15N chemical shifts	45
1H chemical shifts	323
heteronuclear NOE values	38
H exchange rates	44
T1 relaxation values	38
T2 relaxation values	38

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	monomer 1	1
2	monomer 2	1

Entities:

Entity 1, monomer 1 45 residues - Formula weight is not available

1

ARG

ASN

THR

SER

VAL

MET

LYS

GLY

2

ILE

PHE

SER

ALA

GLU

PHE

LEU

LYS

VAL

PHE

3

LEU

PRO

SER

LEU

SER

HIS

LEU

4

ALA

ILE

GLY

LEU

GLY

ILE

TYR

ILE

GLY

ARG

5

ARG

LEU

THR

SER

Samples:

cn_labelled: BNIP3 TM, [U-95% 13C; U-95% 15N], 1.38 ± 0.02 mM; DHPC 44.13 ± 0.02 mM; DMPC 11.03 ± 0.02 mM; Acetic buffer 20 mM; NaN3 1.5 mM; EDTA 1 mM

n_labelled: BNIP3 TM, [U-95% 15N], 1.38 ± 0.02 mM; DHPC 44.13 ± 0.02 mM; DMPC 11.03 ± 0.02 mM; Acetic buffer 20 mM; NaN3 1.5 mM; EDTA 1 mM

heterodimer: BNIP3 TM, [U-95% 13C; U-95% 15N], 0.69 ± 0.02 mM; DHPC 44.13 ± 0.02 mM; DMPC 11.03 ± 0.02 mM; Acetic buffer 20 mM; NaN3 1.5 mM; EDTA 1 mM

conditions_1: pH: 5.10; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N HSQC	not available	not available	conditions_1
3D 15N-NOESY	not available	not available	conditions_1
1H13C HSQC	not available	not available	conditions_1
3D 13C-NOESY	not available	not available	conditions_1
HCCH-TOCSY	not available	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Varian UNITY 600 MHz

BMRB	16012
PDB	2J5D 2KA1 2KA2
DBJ	BAB29214 BAC36072 BAD96346 BAE31356 BAG58159
EMBL	CAX36485
GB	AAC00022 AAC16738 AAD02922 AAF98317 AAH21989
REF	NP_001015859 NP_001069834 NP_001083178 NP_004043 NP_033890
SP	O55003 Q12983 Q32KN2
TPG	DAA14630
AlphaFold	O55003 Q12983 Q32KN2