BMRB Entry 7266

Title:
Chemical shift assignments for protein NE1680 from Nitrosomonas europaea: Northeast Structural Genomics Consortium target NeT5
Deposition date:
2006-08-15
Original release date:
2006-09-05
Authors:
Cort, John; Yee, Adelinda; Arrowsmith, Cheryl; Kennedy, Michaeal
Citation:

Citation: Cort, John; Ramelot, Theresa; Yee, Adelinda; Arrowsmith, Cheryl; Kennedy, Michaeal. "Chemical shift assignments for protein NE1680 from Nitrosomonas europaea: Northeast Structural Genomics Consortium target NeT5"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
NE1680, polymer, 109 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Nitrosomonas europaea   Taxonomy ID: 915   Superkingdom: Eubacteria   Kingdom: Not applicable   Genus/species: Nitrosomonas europaea

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts362
15N chemical shifts87
1H chemical shifts569

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NE16801

Entities:

Entity 1, NE1680 109 residues - Formula weight is not available

The construct has a non-native 22-residue N-term tag sequence (MGSSHHHHHHSSGRENLYFQGH), and a two-residue C-term cloning artifact (GS).

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYARGGLUASNLEUTYRPHEGLN
3   GLYHISMETGLNILEHISVALTYRASPTHR
4   TYRVALLYSALALYSASPGLYHISVALMET
5   HISPHEASPVALPHETHRASPVALARGASP
6   ASPLYSLYSALAILEGLUPHEALALYSGLN
7   TRPLEUSERSERILEGLYGLUGLUGLYALA
8   THRVALTHRSERGLUGLUCYSARGPHECYS
9   HISSERGLULYSALAPROASPGLUVALILE
10   GLUALAILELYSGLNASNGLYTYRPHEILE
11   TYRLYSMETGLUGLYCYSASNGLYSER

Related Database Links:

PDB 2HFQ
EMBL CAD85591
REF NP_841712 WP_011112234

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks