BMRB Entry 7219

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7219

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Title:
NMR Data for the Human Ubiquitin-Conjugating Enzyme Variant hUev1a
Deposition date:
2006-07-10
Original release date:
2006-10-19
Authors:
Hau, David; Lewis, Michael; Saltibus, Linda; Spyracopoulos, Leo
Citation:

Citation: Hau, David; Lewis, Michael; Saltibus, Linda; Pastushok, Landon; Xiao, Wei; Spyracopoulos, Leo. "Structure and interactions of the ubiquitin-conjugating enzyme variant human Uev1a: implications for enzymatic synthesis of polyubiquitin chains"  Biochemistry 45, 9866-9877 (2006).
PubMed: 16893187

Assembly members:

Assembly members:
hUev1a, polymer, 170 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pGEX-6P

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hUev1a: MPGEVQASYLKSQSKLSDEG RLEPRKFHCKGVKVPRNFRL LEELEEGQKGVGDGTVSWGL EDDEDMTLTRWTGMIIGPPR TIYENRIYSLKIECGPKYPE APPFVRFVTKINMNGVNSSN GVVDPRAISVLAKWQNSYSI KVVLQELRRLMMSKENMKLP QPPEGQCYSN

Data sets:
Data typeCount
13C chemical shifts599
15N chemical shifts170
1H chemical shifts1129
coupling constants56
S2 parameters111
T1 relaxation values248
T2 relaxation values249

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1hUev1a1

Entities:

Entity 1, hUev1a 170 residues - Formula weight is not available

1   METPROGLYGLUVALGLNALASERTYRLEU
2   LYSSERGLNSERLYSLEUSERASPGLUGLY
3   ARGLEUGLUPROARGLYSPHEHISCYSLYS
4   GLYVALLYSVALPROARGASNPHEARGLEU
5   LEUGLUGLULEUGLUGLUGLYGLNLYSGLY
6   VALGLYASPGLYTHRVALSERTRPGLYLEU
7   GLUASPASPGLUASPMETTHRLEUTHRARG
8   TRPTHRGLYMETILEILEGLYPROPROARG
9   THRILETYRGLUASNARGILETYRSERLEU
10   LYSILEGLUCYSGLYPROLYSTYRPROGLU
11   ALAPROPROPHEVALARGPHEVALTHRLYS
12   ILEASNMETASNGLYVALASNSERSERASN
13   GLYVALVALASPPROARGALAILESERVAL
14   LEUALALYSTRPGLNASNSERTYRSERILE
15   LYSVALVALLEUGLNGLULEUARGARGLEU
16   METMETSERLYSGLUASNMETLYSLEUPRO
17   GLNPROPROGLUGLYGLNCYSTYRSERASN

Related Database Links:

PDB 2A4D 2C2V 2HLW
DBJ BAK63082
EMBL CAH92507 CAH93170
GB AAB04629 AAB72015 AAC02755 AAG24229 AAH00468
REF NP_001027459 NP_001030574 NP_001128950 NP_001192006 NP_001192013
SP Q13404 Q3SZ52 Q5R4Z6 Q90879
TPG DAA23316
AlphaFold Q13404 Q3SZ52 Q5R4Z6 Q90879

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks