BMRB Entry 7192
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR7192
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Citation: Ramelot, Theresa; Kennedy, Michael. "NMR structure of YqaI from Bacillus sbutilis" The BMRB entry is the only known published source for the data..
Assembly members:
YqaI, polymer, 72 residues, Formula weight is not available
Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: recombinant technology Vector: PET21
Entity Sequences (FASTA):
YqaI: MVENPMVINNWHDKLTETDV
QIDFYGDEVTPVDDYVIDGG
EIILRENLERYLREQLGFEF
KNAQLEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 306 |
| 15N chemical shifts | 75 |
| 1H chemical shifts | 482 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | YqaI, chain 1 | 1 |
| 2 | YqaI, chain 2 | 1 |
Entities:
Entity 1, YqaI, chain 1 72 residues - Formula weight is not available
C-term: LEHHHHHH
| 1 | MET | VAL | GLU | ASN | PRO | MET | VAL | ILE | ASN | ASN | ||||
| 2 | TRP | HIS | ASP | LYS | LEU | THR | GLU | THR | ASP | VAL | ||||
| 3 | GLN | ILE | ASP | PHE | TYR | GLY | ASP | GLU | VAL | THR | ||||
| 4 | PRO | VAL | ASP | ASP | TYR | VAL | ILE | ASP | GLY | GLY | ||||
| 5 | GLU | ILE | ILE | LEU | ARG | GLU | ASN | LEU | GLU | ARG | ||||
| 6 | TYR | LEU | ARG | GLU | GLN | LEU | GLY | PHE | GLU | PHE | ||||
| 7 | LYS | ASN | ALA | GLN | LEU | GLU | HIS | HIS | HIS | HIS | ||||
| 8 | HIS | HIS |
Samples:
sample_1: YqaI, [U-100% 13C; U-100% 15N], 1 mM; MES 20 mM; NaCL 100 mM; DTT 10 mM
conditions_1: ionic strength: 100 mM; pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N NOESY | sample_1 | not available | conditions_1 |
| 13C NOESY (aliph) | sample_1 | not available | conditions_1 |
| 4D NOESY CC HSQC | sample_1 | not available | conditions_1 |
| 15N NOESY in D2O | sample_1 | not available | conditions_1 |
| 13C NOESY in D2O (aliph) | sample_1 | not available | conditions_1 |
| 13C NOESY (arom) | sample_1 | not available | conditions_1 |
| 13C NOESY in D2O (arom) | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian Inova 600 MHz
- Varian Inova 750 MHz
Related Database Links:
| PDB | |
| DBJ | BAA06923 BAA12384 BAM58696 |
| EMBL | CAB14571 CEI57855 CEJ78277 |
| GB | ADV93390 AGG62031 AHA78516 AIC41086 AIC45318 |
| REF | NP_390507 WP_003229905 WP_021480131 YP_004204417 YP_007534614 |
| SP | P45906 |
| AlphaFold | P45906 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks