BMRB Entry 7055

Name: 1H, 13C, and 15N Chemical Shift Assignments for PTH from Mycobacterium tuberculosis H37Rv
Published: 2006-08-07

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7055

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N Chemical Shift Assignments for PTH from Mycobacterium tuberculosis H37Rv

Deposition date:

2006-04-07

Original release date:

2006-08-07

Authors:

Bal, Naresh; Agrawal, Himanshu; Meher, Akshaya; Pulavarti, Surya; Jain, Anupam; Kelly, Geoff; Frenkiel, Thomas; Pastore, Annalisa; Arora, Ashish

Citation:

Citation: Bal, Naresh; Agrawal, Himanshu; Meher, Akshaya; Pulavarti, Surya; Jain, Anupam; Kelly, Geoff; Frenkiel, Thomas; Pastore, Annalisa; Arora, Ashish. "NMR assignment of peptidyl-tRNA hydrolase from Mycobacterium tuberculosis H37Rv" J. Biomol. NMR 36, 53-53 (2006).
PubMed: 16821126

Assembly members:

Assembly members:
PTH, polymer, 191 residues, 20455.3301 Da.

Natural source:

Natural source: Common Name: Mycobacterium tuberculosis H37Rv Taxonomy ID: 83332 Superkingdom: Eubacteria Kingdom: not available Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source: Production method: recombinant technology Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PTH: MAEPLLVVGLGNPGANYART RHNLGFVVADLLAARLGAKF KAHKRSGAEVATGRSAGRSL VLAKPRCYMNESGRQIGPLA KFYSVAPANIIVIHDDLDLE FGRIRLKIGGGEGGHNGLRS VVAALGTKDFQRVRIGIGRP PGRKDPAAFVLENFTPAERA EVPTICEQAADATELLIEQG MEPAQNRVHAW

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	730
15N chemical shifts	171
1H chemical shifts	1058

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PTH monomer	1

Entities:

Entity 1, PTH monomer 191 residues - 20455.3301 Da.

1

MET

ALA

GLU

PRO

LEU

VAL

GLY

LEU

2

GLY

ASN

PRO

GLY

ALA

ASN

TYR

ALA

ARG

THR

3

ARG

HIS

ASN

LEU

GLY

PHE

VAL

ALA

ASP

4

LEU

ALA

ARG

LEU

GLY

ALA

LYS

PHE

5

LYS

ALA

HIS

LYS

ARG

SER

GLY

ALA

GLU

VAL

6

ALA

THR

GLY

ARG

SER

ALA

GLY

ARG

SER

LEU

7

VAL

LEU

ALA

LYS

PRO

ARG

CYS

TYR

MET

ASN

8

GLU

SER

GLY

ARG

GLN

ILE

GLY

PRO

LEU

ALA

9

LYS

PHE

TYR

SER

VAL

ALA

PRO

ALA

ASN

ILE

10

ILE

VAL

ILE

HIS

ASP

LEU

ASP

LEU

GLU

11

PHE

GLY

ARG

ILE

ARG

LEU

LYS

ILE

GLY

12

GLY

GLU

GLY

HIS

ASN

GLY

LEU

ARG

SER

13

VAL

ALA

LEU

GLY

THR

LYS

ASP

PHE

14

GLN

ARG

VAL

ARG

ILE

GLY

ILE

GLY

ARG

PRO

15

PRO

GLY

ARG

LYS

ASP

PRO

ALA

PHE

VAL

16

LEU

GLU

ASN

PHE

THR

PRO

ALA

GLU

ARG

ALA

17

GLU

VAL

PRO

THR

ILE

CYS

GLU

GLN

ALA

18

ASP

ALA

THR

GLU

LEU

ILE

GLU

GLN

GLY

19

MET

GLU

PRO

ALA

GLN

ASN

ARG

VAL

HIS

ALA

20

TRP

Samples:

sample_1: PTH, [U-15N; U-13C], 0.8 mM; sodium phosphate 20 mM; sodium chloride 50 mM; NaN3 0.1%

conditions_1: ionic strength: 50 mM; pH: 6.5; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N HSQC	sample_1	not available	conditions_1
HNCA	sample_1	not available	conditions_1
HNCACB	sample_1	not available	conditions_1
CBCA(CO)NH	sample_1	not available	conditions_1
HCCH-TOCSY	sample_1	not available	conditions_1
H(CCCO)NH-TOCSY	sample_1	not available	conditions_1
(H)CC(CO)NH-TOCSY	sample_1	not available	conditions_1
HNCO	sample_1	not available	conditions_1
HN(CA)CO	sample_1	not available	conditions_1
15N-edited NOESY-HSQC	sample_1	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Varian UnityINOVA 600 MHz
Bruker Avance 600 MHz
Varian UnityINOVA 800 MHz

PDB	2JRC 2Z2I 2Z2J 2Z2K 3TCK 3TCN 3TD2 3TD6
DBJ	BAH25334 BAL64931 BAQ04946 GAA44770
EMBL	CAD93902 CAL71058 CCC26096 CCC43355 CCC63630
GB	AAK45293 ABQ72757 ABR05375 ACT26062 AEB05122
REF	NP_215530 NP_335479 NP_854698 WP_003405251 WP_003916286
SP	A1KHF2 A5U157 C1AM05 P65866 P9WHN6
AlphaFold	A1KHF2 A5U157 C1AM05 P65866 P9WHN6