BMRB Entry 7034

Name: 1H, 13C, and 15N chemical shift assignments for the zinc-finger region of human ZHX1
Published: 2006-04-05

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PDB ID: 2ghf
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7034
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N chemical shift assignments for the zinc-finger region of human ZHX1

Deposition date:

2006-03-27

Original release date:

2006-04-05

Authors:

Wienk, Hans; Lammers, Ivonne; Wu, Jin; Hotze, Anna; Wechselberger, Rainer; Kaptein, Rob; Folkers, Gert

Citation:

Citation: Wienk, Hans; Lammers, Ivonne; Hotze, Anna; Wu, Jin; Wechselberger, Rainer; Owens, Ray; Stammers, David; Stuart, David; Kaptein, Robert; Folkers, Gert. "The tandem zinc-finger region of human ZHX adopts a novel C2H2 zinc finger structure with a C-terminal extension." Biochemistry 48, 4431-4439 (2009).
PubMed: 19348505

Assembly members:

Assembly members:
hZHX1-ZNF, polymer, 102 residues, 12110 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hZHX1-ZNF: MAHHHHHHNQQNKKVEGGYE CKYCTFQTPDLNMFTFHVDS EHPNVVLNSSYVCVECNFLT KRYDALSEHNLKYHPGEENF KLTMVKRNNQTIFEQTINDL TF

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	309
15N chemical shifts	86
1H chemical shifts	532

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	polypeptide	1
2	zinc1	2
3	zinc2	2

Entities:

Entity 1, polypeptide 102 residues - 12110 Da.

1

MET

ALA

HIS

ASN

GLN

2

GLN

ASN

LYS

VAL

GLU

GLY

TYR

GLU

3

CYS

LYS

TYR

CYS

THR

PHE

GLN

THR

PRO

ASP

4

LEU

ASN

MET

PHE

THR

PHE

HIS

VAL

ASP

SER

5

GLU

HIS

PRO

ASN

VAL

LEU

ASN

SER

6

TYR

VAL

CYS

VAL

GLU

CYS

ASN

PHE

LEU

THR

7

LYS

ARG

TYR

ASP

ALA

LEU

SER

GLU

HIS

ASN

8

LEU

LYS

TYR

HIS

PRO

GLY

GLU

ASN

PHE

9

LYS

LEU

THR

MET

VAL

LYS

ARG

ASN

GLN

10

THR

ILE

PHE

GLU

GLN

THR

ILE

ASN

ASP

LEU

11

THR

PHE

Entity 2, zinc1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: hZHX1-ZNF, [U-98% 13C; U-99% 15N], 0.3 mM; NaPO4 30 mM; NaCl 100 mM; ZnCl2 50 microM; D2O 10%

conditions_1: pH: 8.0; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N_HSQC	sample_1	not available	conditions_1
CBCA(CO)NH	sample_1	not available	conditions_1
HNCACB	sample_1	not available	conditions_1
HNCO	sample_1	not available	conditions_1
HN(CA)HA	sample_1	not available	conditions_1
CC(CO)NH	sample_1	not available	conditions_1
H(C)CH-TOCSY	sample_1	not available	conditions_1
(H)CCH-COSY	sample_1	not available	conditions_1
HNH-NOESY	sample_1	not available	conditions_1
HH-NOESY	sample_1	not available	conditions_1

Software:

SPARKY v3, T. D. Goddard and D. G. Kneller - assignment

XWIN-NMR v3.5, BRUKER - processing

NMR spectrometers:

Bruker Avance 700.13 MHz
Bruker Avance 900.21 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks