BMRB Entry 6936

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6936

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Title:
Resonance assignments for the pKM101 homologue of VirB7 (TraN) in complex with the pKM101 homologue of VirB9 (TraO)
Deposition date:
2005-01-13
Original release date:
2006-04-14
Authors:
Harris, Richard; Bayliss, Richard; Waksman, Gabriel; Driscoll, Paul
Citation:

Citation: Harris, Richard; Bayliss, Richard; Waksman, Gabriel; Driscoll, Paul. "Resonance Assignments of the Complex between TraN and the C-terminal Domain of TraO from the Conjugative Plasmid pKM101"  J. Biomol. NMR 36, 31-31 (2006).
PubMed: 16601857

Assembly members:

Assembly members:
VirB7, polymer, 36 residues, 3772 Da.
VirB9, polymer, 126 residues, 14060 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
VirB7: GAMASGHKPPPEPDWSNTVP VNKTIPVDTQGGRNES
VirB9: GIDPFTHMAGAKNYQYVMSE QPEMRSIQPVHVWDNYRFTR FEFPANAELPQVYMISASGK ETLPNSHVVGENRNIIEVET VAKEWRIRLGDKVVGVRNNN FAPGAGAVATGTASPDVRRV QIGEDN

Data sets:
Data typeCount
13C chemical shifts634
15N chemical shifts151
1H chemical shifts1016

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1VirB71
2VirB92

Entities:

Entity 1, VirB7 36 residues - 3772 Da.

GAMA is part of the tag and no chemical shifts for the tag residues are reported. First VirB7 amino acid is S5 which in the proper sequence is residue number 17 as given in the chemical shift list.

1   GLYALAMETALASERGLYHISLYSPROPRO
2   PROGLUPROASPTRPSERASNTHRVALPRO
3   VALASNLYSTHRILEPROVALASPTHRGLN
4   GLYGLYARGASNGLUSER

Entity 2, VirB9 126 residues - 14060 Da.

GIDPFTHM is part of the tag and no chemical shifts for the tag residues are reported. First VirB9 amino acid is A9 which in the proper sequence is residue number 177 as given in the chemical shift list.

1   GLYILEASPPROPHETHRHISMETALAGLY
2   ALALYSASNTYRGLNTYRVALMETSERGLU
3   GLNPROGLUMETARGSERILEGLNPROVAL
4   HISVALTRPASPASNTYRARGPHETHRARG
5   PHEGLUPHEPROALAASNALAGLULEUPRO
6   GLNVALTYRMETILESERALASERGLYLYS
7   GLUTHRLEUPROASNSERHISVALVALGLY
8   GLUASNARGASNILEILEGLUVALGLUTHR
9   VALALALYSGLUTRPARGILEARGLEUGLY
10   ASPLYSVALVALGLYVALARGASNASNASN
11   PHEALAPROGLYALAGLYALAVALALATHR
12   GLYTHRALASERPROASPVALARGARGVAL
13   GLNILEGLYGLUASPASN

Related Database Links:

PDB 2OFQ 3ZBI
DBJ BAF38206 BAF93109 BAM29017
EMBL CCA62524 CCE73953 CCN79904 CDF32038 CDK67343
GB AAA86458 AAL13392 ABG33818 ABG49204 ABO42055
REF NP_511196 WP_000735066 WP_000735067 WP_010999963 WP_013279390

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks