BMRB Entry 6920

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Mid1 BBox 1
Published: 2006-04-27

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PDB ID: 2ffw
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6920
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Mid1 BBox 1

Deposition date:

2005-12-08

Original release date:

2006-04-27

Authors:

Massiah, Michael; Simmons, Brandi; Short, Keiran; Cox, Tim

Citation:

Citation: Massiah, Michael; Simmons, Brandi; Short, Keiran; Cox, Tim. "Solution Structure of the RBCC/TRIM B-box1 Domain of Human MID1: B-box with a RING" J. Mol. Biol. 358, 532-545 (2006).
PubMed: 16529770

Assembly members:

Assembly members:
B-box 1 type 1, polymer, 78 residues, 8620 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Vector: pGEX 4T-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
B-box 1 type 1: QKASVSGPNSPSETRRERAF DANTMTSAEKVLCQFCDQDP AQDAVKTCVTCEVSYCDECL KATHPNKKPFTGHRLIEP

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	174
15N chemical shifts	73
1H chemical shifts	421

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Midline-1 B-box Type 1	1
2	ZINC (II) ION, 1	2
3	ZINC (II) ION, 2	2

Entities:

Entity 1, Midline-1 B-box Type 1 78 residues - 8620 Da.

Sequence corresponds to Gln87 to Pro164 in Midline1

1

GLN

LYS

ALA

SER

VAL

SER

GLY

PRO

ASN

SER

2

PRO

SER

GLU

THR

ARG

GLU

ARG

ALA

PHE

3

ASP

ALA

ASN

THR

MET

THR

SER

ALA

GLU

LYS

4

VAL

LEU

CYS

GLN

PHE

CYS

ASP

GLN

ASP

PRO

5

ALA

GLN

ASP

ALA

VAL

LYS

THR

CYS

VAL

THR

6

CYS

GLU

VAL

SER

TYR

CYS

ASP

GLU

CYS

LEU

7

LYS

ALA

THR

HIS

PRO

ASN

LYS

PRO

PHE

8

THR

GLY

HIS

ARG

LEU

ILE

GLU

PRO

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: B-box 1 type 1, [U-13C; U-15N], 0.75 ± 0.1 mM; ZINC (II) ION mM; Tris 50 mM; NaCl 300 mM

conditions_1: pH: 7.5; temperature: 294 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N_HSQC	sample_1	not available	conditions_1
HNCA	sample_1	not available	conditions_1
HNCACB	sample_1	not available	conditions_1
CBCACONH	sample_1	not available	conditions_1
HMQC-NOE-HSQC	sample_1	not available	conditions_1
15N-HSQC-NOESY	sample_1	not available	conditions_1
13C-HSQC-NOESY	sample_1	not available	conditions_1
HCCH-TOCSY	sample_1	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Varian Inova 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks