BMRB Entry 6907

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6907
MolProbity Validation Chart

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Title:
Chemical Shift Assignments for V66W110 fragment of Staphylococcal Nuclease
Deposition date:
2005-11-22
Original release date:
2007-07-10
Authors:
Liu, Dongsheng; Xie, Tao; Feng, Yingang; Shan, Lu; Ye, Keqiong; Wang, Jinfeng
Citation:

Citation: Xie, Tao; Liu, Dongsheng; Feng, Yingang; Shan, Lu; Wang, Jinfeng. "Folding stability and cooperativity of the three forms of 1-110 residues fragment of staphylococcal nuclease"  Biophys. J. 92, 2090-2107 (2007).
PubMed: 17172296

Assembly members:

Assembly members:
V66W110 fragment of staphylococcal nuclease, polymer, 110 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
V66W110 fragment of staphylococcal nuclease: ATSTKKLHKEPATLIKAIDG DTVKLMYKGQPMTFRLLLVD TPETKHPKKGVEKYGPEASA FTKKMWENAKKIEVEFDKGQ RTDKYGRGLAYIYADGKMVN EALVRQGLAK

Data sets:
Data typeCount
15N chemical shifts91
1H chemical shifts481

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1V66W110 fragment of staphylococcal nuclease1

Entities:

Entity 1, V66W110 fragment of staphylococcal nuclease 110 residues - Formula weight is not available

1   ALATHRSERTHRLYSLYSLEUHISLYSGLU
2   PROALATHRLEUILELYSALAILEASPGLY
3   ASPTHRVALLYSLEUMETTYRLYSGLYGLN
4   PROMETTHRPHEARGLEULEULEUVALASP
5   THRPROGLUTHRLYSHISPROLYSLYSGLY
6   VALGLULYSTYRGLYPROGLUALASERALA
7   PHETHRLYSLYSMETTRPGLUASNALALYS
8   LYSILEGLUVALGLUPHEASPLYSGLYGLN
9   ARGTHRASPLYSTYRGLYARGGLYLEUALA
10   TYRILETYRALAASPGLYLYSMETVALASN
11   GLUALALEUVALARGGLNGLYLEUALALYS

Samples:

sample_1: V66W110 fragment of staphylococcal nuclease, [U-95% 15N], 1 – 2.5 mM; deuterated acetate, [U-99% 2H], 50 ± 1 mM

conditions_1: pH: 4.9; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N-HSQCsample_1not availableconditions_1
1H-15N TOCSY-HSQCsample_1not availableconditions_1
1H-15N NOESY-HSQCsample_1not availableconditions_1

Software:

FELIX v980 -

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 136 15357 1581 1582 16585 1704 17718 18013 1874 1875 1876 1877 1878 18788 188 189 2784 2785 4010 4052 4053 4905 494 495 496 497 530 5536 6250 6251 644 6908
PDB
DBJ BAB41979 BAB56977 BAB94634 BAF67032 BAF77694
EMBL CAA24594 CAG39855 CAG42530 CAI80436 CAQ49298
GB AAC14660 AAW36415 ABD22328 ABD29945 ABE02272
PRF 1109959A 710414A
REF NP_371339 NP_374001 NP_645586 WP_000141556 WP_000141557
SP P00644 Q5HHM4 Q6GB41 Q6GIK1 Q7A6P2
AlphaFold P00644 Q5HHM4 Q6GB41 Q6GIK1 Q7A6P2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks