BMRB Entry 6876

Title:
1H, 15N and 13C chemical shifts of T. cruzi Chagasin
Deposition date:
2005-10-25
Original release date:
2007-01-29
Authors:
Aido-Machado, Rodolpho do; Salmon, Didier; Diehl, Anne; Leidert, Martina; Schmetzer, Oliver; Lima, Ana; Scharfstein, Julio; Oschkinat, Hartmut; Pires, Jose
Citation:

Citation: Aido-Machado, Rodolpho do; Salmon, Didier; Diehl, Anne; Leidert, Martina; Schmetzer, Oliver; Lima, Ana; Scharfstein, Julio; Oschkinat, Hartmut; Pires, Jose. "1H, 15N and 13C assignments of the cysteine protease inhibitor Chagasin from Trypanosoma cruzi"  J. Biomol. NMR 36, 30-30 (2006).
PubMed: 16680406

Assembly members:

Assembly members:
Chagasin, polymer, 111 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Trypanosoma cruzi   Taxonomy ID: 5693   Superkingdom: Eukaryota   Kingdom: Protista   Genus/species: Trypanosoma cruzi

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts335
15N chemical shifts113
1H chemical shifts648

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Chagasin1

Entities:

Entity 1, Chagasin 111 residues - Formula weight is not available

1   GLYSERSERHISLYSVALTHRLYSALAHIS
2   ASNGLYALATHRLEUTHRVALALAVALGLY
3   GLULEUVALGLUILEGLNLEUPROSERASN
4   PROTHRTHRGLYPHEALATRPTYRPHEGLU
5   GLYGLYTHRLYSGLUSERPROASNGLUSER
6   METPHETHRVALGLUASNLYSTYRPHEPRO
7   PROASPSERLYSLEULEUGLYALAGLYGLY
8   THRGLUHISPHEHISVALTHRVALLYSALA
9   ALAGLYTHRHISALAVALASNLEUTHRTYR
10   METARGPROTRPTHRGLYPROSERHISASP
11   SERGLUARGPHETHRVALTYRLEULYSALA
12   ASN

Samples:

sample_1: Chagasin, [U-13C; U-15N], 0.3 mM; Phosphate Buffer 20 mM; NaCl 50 mM

sample_2: Chagasin, [U-13C; U-15N], 0.7 mM; Phosphate Buffer 20 mM; NaCl 50 mM

sample_3: Chagasin, [U-15N], 0.5 mM; Phosphate Buffer 20 mM; NaCl 50 mM

sample_4: Chagasin 1.2 mM; Phosphate Buffer 20 mM; NaCl 50 mM

conditions_1: pH: 5.6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
triple-resonance experimentsnot availablenot availableconditions_1
13C_NOESY HMQCnot availablenot availableconditions_1
15N NOESY HSQCnot availablenot availableconditions_1
2D NOESYnot availablenot availableconditions_1
2D TOCSYnot availablenot availableconditions_1
1H15N_HSQCnot availablenot availableconditions_1

Software:

ARIA v1.2, Michael Nilges et al. -

NMR spectrometers:

    Related Database Links:

    BMRB 16421
    PDB
    EMBL CAC39242
    SP Q966X9
    AlphaFold Q966X9

    Download HSQC peak lists in one of the following formats:
    CSV: Backbone or all simulated peaks
    SPARKY: Backbone or all simulated peaks