BMRB Entry 6845

Name: NMR Structural analysis of archaeal Nop10
Published: 2005-12-28

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PDB ID: 2aqc
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6845
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Structural analysis of archaeal Nop10

Deposition date:

2005-09-29

Original release date:

2005-12-28

Authors:

Hamma, T.; Reichow, S.; Varani, G.; Ferre-D'Amare, A.

Citation:

Citation: Hamma, T.; Reichow, S.; Varani, G.; Ferre-D'Amare, A.. "The Cbf5-Nop10 complex is a molecular bracket that organizes box H/ACA RNPs" Nat. Struct. Mol. Biol. 12, 1101-1107 (2005).
PubMed: 16286935

Assembly members:

Assembly members:
Ribosome biogenesis protein Nop10, polymer, 63 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Methanocaldococcus jannaschii Taxonomy ID: 2190 Superkingdom: Archaea Kingdom: not available Genus/species: Methanocaldococcus jannaschii

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ribosome biogenesis protein Nop10: GSHMVEMRMKKCPKCGLYTL KEICPKCGEKTVIPKPPKFS LEDRWGKYRRMLKRALKNKN KAE

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	273
15N chemical shifts	64
1H chemical shifts	480

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ribosome biogenesis protein Nop10	1
2	ZINC (II) ION	2

Entities:

Entity 1, Ribosome biogenesis protein Nop10 63 residues - Formula weight is not available

1

GLY

SER

HIS

MET

VAL

GLU

MET

ARG

MET

LYS

2

LYS

CYS

PRO

LYS

CYS

GLY

LEU

TYR

THR

LEU

3

LYS

GLU

ILE

CYS

PRO

LYS

CYS

GLY

GLU

LYS

4

THR

VAL

ILE

PRO

LYS

PRO

LYS

PHE

SER

5

LEU

GLU

ASP

ARG

TRP

GLY

LYS

TYR

ARG

6

MET

LEU

LYS

ARG

ALA

LEU

LYS

ASN

LYS

ASN

7

LYS

ALA

GLU

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: Ribosome biogenesis protein Nop10 1 mM; Sodium Acetate 50 mM; KCl 100 mM; DTT 2 mM; ZnCl2 100 uM; H2O 90%; D2O 10%

sample_2: Ribosome biogenesis protein Nop10 1 mM; Sodium Acetate 50 mM; KCl 100 mM; DTT 2 mM; ZnCl2 100 uM; D2O 100%

sample_3: Ribosome biogenesis protein Nop10, [U-15N], 1 mM; Sodium Acetate 50 mM; KCl 100 mM; DTT 2 mM; ZnCl2 100 uM; H2O 90%; D2O 10%

sample_4: Ribosome biogenesis protein Nop10, [U-13C; U-15N], 1 mM; Sodium Acetate 50 mM; KCl 100 mM; DTT 2 mM; ZnCl2 100 uM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 100 mM; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	sample_cond_1
3D 15N-separated NOESY	not available	not available	sample_cond_1
3D 13C-separated NOESY	not available	not available	sample_cond_1

Software:

CYANA vv. 2.0, Guntert, P. - refinement

NMRPipe vv. 2.3, Delaglio, F. - processing

SPARKY vv. 3.110, Goddard. T.D. - data analysis

TALOS vv. 2003.027.13.05, Cornilescu, G. - data analysis

NMR spectrometers:

Varian INOVA 800 MHz

PDB	2AQC
GB	AAB98109
REF	NP_247081 WP_010869609
SP	P81303
AlphaFold	P81303