BMRB Entry 6824

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6824

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Title:
1H, 13C, and 15N Chemical Shift Assignments for ORF 7a coded X4 protein of SARS Coronavirus
Deposition date:
2005-09-13
Original release date:
2007-01-29
Authors:
Stangler, Thomas; Stoldt, Matthias; Willbold, Dieter
Citation:

Citation: Hanel, Karen; Stangler, Thomas; Stoldt, Matthias; Willbold, Dieter. "Solution structure of the X4 protein coded by the SARS related coronavirus reveals an immunoglobulin like fold and suggests a binding activity to integrin I domains."  J. Biomed. Sci. 13, 281-293 (2005).
PubMed: 16328780

Assembly members:

Assembly members:
SARS CoV 7a protein, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: SARS Coronavirus   Taxonomy ID: 227859   Superkingdom: Viruses   Kingdom: Not applicable   Genus/species: Coronavirus SARS Coronavirus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SARS CoV 7a protein: GPELYHYQECVRGTTVLLKE PCPSGTYEGNSPFHPLADNK FALTCTSTHFAFACADGTRH TYQLRARSVSPKLFIRQEEV QQELYSR

Data sets:
Data typeCount
13C chemical shifts350
15N chemical shifts80
1H chemical shifts524

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SARS CoV 7a protein1

Entities:

Entity 1, SARS CoV 7a protein 87 residues - Formula weight is not available

1   GLYPROGLULEUTYRHISTYRGLNGLUCYS
2   VALARGGLYTHRTHRVALLEULEULYSGLU
3   PROCYSPROSERGLYTHRTYRGLUGLYASN
4   SERPROPHEHISPROLEUALAASPASNLYS
5   PHEALALEUTHRCYSTHRSERTHRHISPHE
6   ALAPHEALACYSALAASPGLYTHRARGHIS
7   THRTYRGLNLEUARGALAARGSERVALSER
8   PROLYSLEUPHEILEARGGLNGLUGLUVAL
9   GLNGLNGLULEUTYRSERARG

Samples:

sample_1: SARS CoV 7a protein, [U-95% 13C; U-98% 15N], 0.4 mM; Sodium Acetate, [U-2H], 1 mM

conditions_1: ionic strength: 5 mM; pH: 5; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
H(CCO)NH-TOCSYsample_1not availableconditions_1
C(CO)NH-TOCSYsample_1not availableconditions_1
HCCH-COSYsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
1H-13C-HSQC-NOESYsample_1not availableconditions_1

Software:

NMRPipe - Processing

CARA v1.1 - data evaluation

NMR spectrometers:

  • Varian Unity INOVA 600 MHz
  • Varian Unity Inova 800 MHz

Related Database Links:

PDB
DBJ BAC81354 BAC81368 BAC81382 BAC81396 BAC81410
GB AAP13449 AAP13573 AAP30036 AAP33703 AAP37023
REF NP_828857
SP P59635 Q3I5J0 Q3LZX7
AlphaFold P59635 Q3I5J0 Q3LZX7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks