BMRB Entry 6763

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6763
MolProbity Validation Chart

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Title:
1H, 15N and 13C resonance assignments of the middle domain of human release factor eRF1
Deposition date:
2005-08-08
Original release date:
2006-03-10
Authors:
Polshakov, Vladimi; Birdsall, Berry; Ivanova, Elena; Kisselev, Lev
Citation:

Citation: Ivanova, Elena; Kolosov, Peter; Birdsall, Berry; Kisselev, Lev; Polshakov, Vladimir. "NMR Assignments of the Middle Domain of Human Polypeptide Release Factor eRF1"  J. Biomol. NMR 36, 8-8 (2006).
PubMed: 16502324

Assembly members:

Assembly members:
middle domain of human release factor eRF1, polymer, 136 residues, 14947 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET23b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
middle domain of human release factor eRF1: LSDDSKFGFIVIDGSGALFG TLQGNTREVLHKFTVDLPKK HGRGGQSALRFARLRMEKRH NYVRKVAETAVQLFISGDKV NVAGLVLAGSADFKTELSQS DMFDQRLQSKVLKLVDISYG GENGFNQAIELSTEVL

Data sets:
Data typeCount
13C chemical shifts459
15N chemical shifts136
1H chemical shifts815

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1middle domain of human eRF11

Entities:

Entity 1, middle domain of human eRF1 136 residues - 14947 Da.

1   LEUSERASPASPSERLYSPHEGLYPHEILE
2   VALILEASPGLYSERGLYALALEUPHEGLY
3   THRLEUGLNGLYASNTHRARGGLUVALLEU
4   HISLYSPHETHRVALASPLEUPROLYSLYS
5   HISGLYARGGLYGLYGLNSERALALEUARG
6   PHEALAARGLEUARGMETGLULYSARGHIS
7   ASNTYRVALARGLYSVALALAGLUTHRALA
8   VALGLNLEUPHEILESERGLYASPLYSVAL
9   ASNVALALAGLYLEUVALLEUALAGLYSER
10   ALAASPPHELYSTHRGLULEUSERGLNSER
11   ASPMETPHEASPGLNARGLEUGLNSERLYS
12   VALLEULYSLEUVALASPILESERTYRGLY
13   GLYGLUASNGLYPHEASNGLNALAILEGLU
14   LEUSERTHRGLUVALLEU

Samples:

sample_1: middle domain of human release factor eRF1, [U-95% 13C; U-95% 15N], 1.2 ± 0.2 mM

sample_2: middle domain of human release factor eRF1, [U-95% 15N], 1.2 ± 0.2 mM

conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCnot availablenot availableconditions_1
1H13C_HSQCnot availablenot availableconditions_1
HNCOnot availablenot availableconditions_1
HNCAnot availablenot availableconditions_1
HN(CO)CAnot availablenot availableconditions_1
HNCACBnot availablenot availableconditions_1
CBCA(CO)NHnot availablenot availableconditions_1
HBHA(CO)NHnot availablenot availableconditions_1
HCCH_TOCSYnot availablenot availableconditions_1
15N_NOESY_HSQCnot availablenot availableconditions_1
13C_NOESY_HSQCnot availablenot availableconditions_1

Software:

NMRPipe v2.3, Frank Delaglio - spectra processing

AutoAssign v1.11.00, G.T. Montelione - automated peak assignments

SPARKY v3.106, T. D. Goddard -

NMR spectrometers:

  • Varian Inova 600 MHz
  • Bruker Avance 600 MHz
  • Varian INOVA 800 MHz
  • Varian Unity Plus 500 MHz

Related Database Links:

BMRB 19506
PDB
DBJ BAA13439 BAA85489 BAC33839 BAE31210 BAE31619
EMBL CAA37987 CAA57281 CAA57282 CAA78620 CAH93389
GB AAA36665 AAB49726 AAD43966 AAH13717 AAH14269
REF NP_001008345 NP_001069722 NP_001076236 NP_001084363 NP_001126989
SP P35615 P62495 P62496 P62497 P62498
TPG DAA27419
AlphaFold P35615 P62495 P62496 P62497 P62498

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks