BMRB Entry 6722
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6722
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Citation: Saxena, Krishna; Elshorst, Bettina; Berk, Holger; Betz, Marco; Grimme, Susanne; Langer, Thomas; Pescatore, Barbara; Schieborr, Ulrich; Vogtherr, Martin; Schwalbe, Harald. "Backbone NMR Assignment of the Low-molecular-weight Protein Tyrosine
Phosphatase (MPtpA) from Mycobacterium Tuberculosis" J. Biomol. NMR 33, 136-136 (2005).
PubMed: 16258834
Assembly members:
Low-Molecular-Weight Protein Tyrosine Phosphatase, polymer, 165 residues, Formula weight is not available
Natural source: Common Name: Mycobacterium tuberculosis Taxonomy ID: 1773 Superkingdom: Eubacteria Kingdom: Not applicable Genus/species: Mycobacterium tuberculosis
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Low-Molecular-Weight Protein Tyrosine Phosphatase: GHMSDPLHVTFVCTGNICRS
PMAEKMFAQQLRHRGLGDAV
RVTSAGTGNWHVGSCADERA
AGVLRAHGYPTDHRAAQVGT
EHLAADLLVALDRNHARLLR
QLGVEAARVRMLRSFDPRSG
THALDVEDPYYGDHSDFEEV
FAVIESALPGLHDWVDERLA
RNGPS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 444 |
| 15N chemical shifts | 147 |
| 1H chemical shifts | 147 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Low-Molecular-Weight Protein Tyrosin Phosphatase | 1 |
Entities:
Entity 1, Low-Molecular-Weight Protein Tyrosin Phosphatase 165 residues - Formula weight is not available
The first two amino acids are due to cloning
| 1 | GLY | HIS | MET | SER | ASP | PRO | LEU | HIS | VAL | THR | ||||
| 2 | PHE | VAL | CYS | THR | GLY | ASN | ILE | CYS | ARG | SER | ||||
| 3 | PRO | MET | ALA | GLU | LYS | MET | PHE | ALA | GLN | GLN | ||||
| 4 | LEU | ARG | HIS | ARG | GLY | LEU | GLY | ASP | ALA | VAL | ||||
| 5 | ARG | VAL | THR | SER | ALA | GLY | THR | GLY | ASN | TRP | ||||
| 6 | HIS | VAL | GLY | SER | CYS | ALA | ASP | GLU | ARG | ALA | ||||
| 7 | ALA | GLY | VAL | LEU | ARG | ALA | HIS | GLY | TYR | PRO | ||||
| 8 | THR | ASP | HIS | ARG | ALA | ALA | GLN | VAL | GLY | THR | ||||
| 9 | GLU | HIS | LEU | ALA | ALA | ASP | LEU | LEU | VAL | ALA | ||||
| 10 | LEU | ASP | ARG | ASN | HIS | ALA | ARG | LEU | LEU | ARG | ||||
| 11 | GLN | LEU | GLY | VAL | GLU | ALA | ALA | ARG | VAL | ARG | ||||
| 12 | MET | LEU | ARG | SER | PHE | ASP | PRO | ARG | SER | GLY | ||||
| 13 | THR | HIS | ALA | LEU | ASP | VAL | GLU | ASP | PRO | TYR | ||||
| 14 | TYR | GLY | ASP | HIS | SER | ASP | PHE | GLU | GLU | VAL | ||||
| 15 | PHE | ALA | VAL | ILE | GLU | SER | ALA | LEU | PRO | GLY | ||||
| 16 | LEU | HIS | ASP | TRP | VAL | ASP | GLU | ARG | LEU | ALA | ||||
| 17 | ARG | ASN | GLY | PRO | SER |
Samples:
sample_1: Low-Molecular-Weight Protein Tyrosine Phosphatase, [U-13C; U-15N], 0.5 mM; Hepes 25 mM; NaCL 150 mM; DTT 10 mM
conditions_1: pH: 7.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| CBCACONH | sample_1 | not available | conditions_1 |
| HNCA | sample_1 | not available | conditions_1 |
| HNCO | sample_1 | not available | conditions_1 |
| HNCACO | sample_1 | not available | conditions_1 |
Software:
CARA v1.3, Rochus Keller, Prof. Kurt Wuthrich - Assignment
NMR spectrometers:
- Bruker DRX 800 MHz
Related Database Links:
| BMRB | 18533 19361 19388 |
| PDB | |
| DBJ | BAH26529 BAL66249 BAQ06307 GAA45923 |
| EMBL | CAD97111 CAL72239 CCC27315 CCC44588 CCC64828 |
| GB | AAK46577 ABQ74015 ABR06594 ACT24805 AEB03885 |
| REF | NP_216750 NP_336763 NP_855907 WP_003411510 WP_003899227 |
| SP | P65717 P9WIA0 P9WIA1 |
| AlphaFold | P65717 P9WIA0 P9WIA1 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks