BMRB Entry 6696

Name: G83N TPR domain of Ppp5
Published: 2006-04-05

Click here to enlarge.

PDB ID: 2bug
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6696
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

G83N TPR domain of Ppp5

Deposition date:

2005-06-21

Original release date:

2006-04-05

Authors:

Cliff, Matthew; Harris, Richard; Barford, David; Ladbury, John; Williams, Mark

Citation:

Citation: Cliff, Matthew; Harris, Richard; Barford, David; Ladbury, John; Williams, Mark. "Conformational Diversity in the TPR Domain-Mediated Interaction of Protein Phosphatase 5 with Hsp90" Structure 14, 415-426 (2006).
PubMed: 16531226

Assembly members:

Assembly members:
tetratricopeptide domain from Ppp5, polymer, 140 residues, 16179 Da.
tetratricopeptide domain from Ppp5, polymer, 6 residues, 621 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Vector: pQE30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
tetratricopeptide domain from Ppp5: MSGHHHHHHTDPPADGALKR AEELKTQANDYFKAKDYENA IKFYSQAIELNPSNAIYYGN RSLAYLRTECYGYALNDATR AIELDKKYIKGYYRRAASNM ALGKFRAALRDYETVVKVKP HDKDAKMKYQECNKIVKQKA
tetratricopeptide domain from Ppp5: XMEEVD

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	563
15N chemical shifts	138
1H chemical shifts	933

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PP5-TPR	1
2	Hsp90 Pentapeptide	2

Entities:

Entity 1, PP5-TPR 140 residues - 16179 Da.

Residues 19 - 147 of Ppp5 with G83N mutation and N-terminal His-tag.

1	MET	SER	GLY	HIS	HIS	HIS	HIS	HIS	HIS	THR
2	ASP	PRO	PRO	ALA	ASP	GLY	ALA	LEU	LYS	ARG
3	ALA	GLU	GLU	LEU	LYS	THR	GLN	ALA	ASN	ASP
4	TYR	PHE	LYS	ALA	LYS	ASP	TYR	GLU	ASN	ALA
5	ILE	LYS	PHE	TYR	SER	GLN	ALA	ILE	GLU	LEU
6	ASN	PRO	SER	ASN	ALA	ILE	TYR	TYR	GLY	ASN
7	ARG	SER	LEU	ALA	TYR	LEU	ARG	THR	GLU	CYS
8	TYR	GLY	TYR	ALA	LEU	ASN	ASP	ALA	THR	ARG
9	ALA	ILE	GLU	LEU	ASP	LYS	LYS	TYR	ILE	LYS
10	GLY	TYR	TYR	ARG	ARG	ALA	ALA	SER	ASN	MET
11	ALA	LEU	GLY	LYS	PHE	ARG	ALA	ALA	LEU	ARG
12	ASP	TYR	GLU	THR	VAL	VAL	LYS	VAL	LYS	PRO
13	HIS	ASP	LYS	ASP	ALA	LYS	MET	LYS	TYR	GLN
14	GLU	CYS	ASN	LYS	ILE	VAL	LYS	GLN	LYS	ALA

Entity 2, Hsp90 Pentapeptide 6 residues - 621 Da.

1

ACE

MET

GLU

VAL

ASP

Name	Sample	Sample state	Sample conditions
1H15NHSQC	sample_1	not available	conditions_1
1H13CHSQC	sample_1	not available	conditions_1
HNCA	sample_1	not available	conditions_1
CBCACONH	sample_1	not available	conditions_1
HNHB	sample_1	not available	conditions_1
1H15NTOWNY HSQC	sample_1	not available	conditions_1
1H15NNOESY HSQC	sample_1	not available	conditions_1
HNCO	sample_1	not available	conditions_1
1H13CNOESY HSQC	sample_1	not available	conditions_1
HCCH-TOCSY-HSQC	sample_1	not available	conditions_1

PDB	1A17 1WAO 2BUG
DBJ	BAD96942 BAE90444 BAG35563 BAI45682
EMBL	CAA61595 CAH91828
GB	AAB60384 AAD22669 AAH00750 AAH01831 AAH01970
REF	NP_001127468 NP_001179178 NP_006238 XP_001111749 XP_001167704
SP	P53041
AlphaFold	P53041

BMRB Entry 6696

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	SER	GLY	HIS	HIS	HIS	HIS	HIS	HIS	THR
2	ASP	PRO	PRO	ALA	ASP	GLY	ALA	LEU	LYS	ARG
3	ALA	GLU	GLU	LEU	LYS	THR	GLN	ALA	ASN	ASP
4	TYR	PHE	LYS	ALA	LYS	ASP	TYR	GLU	ASN	ALA
5	ILE	LYS	PHE	TYR	SER	GLN	ALA	ILE	GLU	LEU
6	ASN	PRO	SER	ASN	ALA	ILE	TYR	TYR	GLY	ASN
7	ARG	SER	LEU	ALA	TYR	LEU	ARG	THR	GLU	CYS
8	TYR	GLY	TYR	ALA	LEU	ASN	ASP	ALA	THR	ARG
9	ALA	ILE	GLU	LEU	ASP	LYS	LYS	TYR	ILE	LYS
10	GLY	TYR	TYR	ARG	ARG	ALA	ALA	SER	ASN	MET
11	ALA	LEU	GLY	LYS	PHE	ARG	ALA	ALA	LEU	ARG
12	ASP	TYR	GLU	THR	VAL	VAL	LYS	VAL	LYS	PRO
13	HIS	ASP	LYS	ASP	ALA	LYS	MET	LYS	TYR	GLN
14	GLU	CYS	ASN	LYS	ILE	VAL	LYS	GLN	LYS	ALA

1	MET	SER	GLY	HIS	HIS	HIS	HIS	HIS	HIS	THR
2	ASP	PRO	PRO	ALA	ASP	GLY	ALA	LEU	LYS	ARG
3	ALA	GLU	GLU	LEU	LYS	THR	GLN	ALA	ASN	ASP
4	TYR	PHE	LYS	ALA	LYS	ASP	TYR	GLU	ASN	ALA
5	ILE	LYS	PHE	TYR	SER	GLN	ALA	ILE	GLU	LEU
6	ASN	PRO	SER	ASN	ALA	ILE	TYR	TYR	GLY	ASN
7	ARG	SER	LEU	ALA	TYR	LEU	ARG	THR	GLU	CYS
8	TYR	GLY	TYR	ALA	LEU	ASN	ASP	ALA	THR	ARG
9	ALA	ILE	GLU	LEU	ASP	LYS	LYS	TYR	ILE	LYS
10	GLY	TYR	TYR	ARG	ARG	ALA	ALA	SER	ASN	MET
11	ALA	LEU	GLY	LYS	PHE	ARG	ALA	ALA	LEU	ARG
12	ASP	TYR	GLU	THR	VAL	VAL	LYS	VAL	LYS	PRO
13	HIS	ASP	LYS	ASP	ALA	LYS	MET	LYS	TYR	GLN
14	GLU	CYS	ASN	LYS	ILE	VAL	LYS	GLN	LYS	ALA

1	MET	SER	GLY	HIS	HIS	HIS	HIS	HIS	HIS	THR
2	ASP	PRO	PRO	ALA	ASP	GLY	ALA	LEU	LYS	ARG
3	ALA	GLU	GLU	LEU	LYS	THR	GLN	ALA	ASN	ASP
4	TYR	PHE	LYS	ALA	LYS	ASP	TYR	GLU	ASN	ALA
5	ILE	LYS	PHE	TYR	SER	GLN	ALA	ILE	GLU	LEU
6	ASN	PRO	SER	ASN	ALA	ILE	TYR	TYR	GLY	ASN
7	ARG	SER	LEU	ALA	TYR	LEU	ARG	THR	GLU	CYS
8	TYR	GLY	TYR	ALA	LEU	ASN	ASP	ALA	THR	ARG
9	ALA	ILE	GLU	LEU	ASP	LYS	LYS	TYR	ILE	LYS
10	GLY	TYR	TYR	ARG	ARG	ALA	ALA	SER	ASN	MET
11	ALA	LEU	GLY	LYS	PHE	ARG	ALA	ALA	LEU	ARG
12	ASP	TYR	GLU	THR	VAL	VAL	LYS	VAL	LYS	PRO
13	HIS	ASP	LYS	ASP	ALA	LYS	MET	LYS	TYR	GLN
14	GLU	CYS	ASN	LYS	ILE	VAL	LYS	GLN	LYS	ALA