BMRB Entry 6682

Name: Solution Structure of a Human C2H2-type Zinc Finger Protein
Published: 2005-07-27

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PDB ID: 1zr9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR6682
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of a Human C2H2-type Zinc Finger Protein

Deposition date:

2005-06-09

Original release date:

2005-07-27

Authors:

Lytle, B.; Peterson, F.; Volkman, B.

Citation:

Citation: Hayes, P.; Lytle, B.; Volkman, B.; Peterson, F.. "The solution structure of ZNF593 from Homo sapiens reveals a zinc finger in a predominantly unstructured protein" Protein Sci. 17, 571-576 (2008).
PubMed: 18287285

Assembly members:

Assembly members:
Zinc finger protein 593, polymer, 124 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Zinc finger protein 593: GHHHHHHLEKAKRRRPDLDE IHRELRPQGSARPQPDPNAE FDPDLPGGGLHRCLACARYF IDSTNLKTHFRSKDHKKRLK QLSVEPYSQEEAERAAGMGS YVPPRRLAVPTEVSTEVPEM DTST

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	404
13C chemical shifts	278
15N chemical shifts	68

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Zinc finger protein 593	1
2	ZINC ION	2

Entities:

Entity 1, Zinc finger protein 593 124 residues - Formula weight is not available

1

GLY

HIS

LEU

GLU

LYS

2

ALA

LYS

ARG

PRO

ASP

LEU

ASP

GLU

3

ILE

HIS

ARG

GLU

LEU

ARG

PRO

GLN

GLY

SER

4

ALA

ARG

PRO

GLN

PRO

ASP

PRO

ASN

ALA

GLU

5

PHE

ASP

PRO

ASP

LEU

PRO

GLY

LEU

6

HIS

ARG

CYS

LEU

ALA

CYS

ALA

ARG

TYR

PHE

7

ILE

ASP

SER

THR

ASN

LEU

LYS

THR

HIS

PHE

8

ARG

SER

LYS

ASP

HIS

LYS

ARG

LEU

LYS

9

GLN

LEU

SER

VAL

GLU

PRO

TYR

SER

GLN

GLU

10

GLU

ALA

GLU

ARG

ALA

GLY

MET

GLY

SER

11

TYR

VAL

PRO

ARG

LEU

ALA

VAL

PRO

12

THR

GLU

VAL

SER

THR

GLU

VAL

PRO

GLU

MET

13

ASP

THR

SER

THR

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: Zinc finger protein 593, [U-15N; U-13C], 0.6 mM; Bis Tris 10 mM; NaCl 100 mM; DTT 2 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.0; temperature: 298 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	sample_1	not available	sample_cond_1
HNCA	sample_1	not available	sample_cond_1
HNCO	sample_1	not available	sample_cond_1
HN(CO)CA	sample_1	not available	sample_cond_1
HNCACB	sample_1	not available	sample_cond_1
HN(CA)CO	sample_1	not available	sample_cond_1
C(CO)NH	sample_1	not available	sample_cond_1
HBHA(CO)NH	sample_1	not available	sample_cond_1
HCCH-TOCSY	sample_1	not available	sample_cond_1
3D 13C-separated NOESY (aliph)	sample_1	not available	sample_cond_1
3D 15N-separated NOESY	sample_1	not available	sample_cond_1
3D 13C-separated NOESY (AROMATIC)	sample_1	not available	sample_cond_1

Software:

XWINNMR v3.5 - collection

NMRPipe v2004 - processing

SPSCAN v1.1.0 - data analysis

XEASY v1.3 - data analysis

GARANT v2.1 - data analysis

CYANA v1.0.6 - structure solution

XPLOR-NIH v2.0.6 - refinement

NMR spectrometers:

Bruker AVANCE 600 MHz

SWISS-PROT	O00488
GenBank	ABZ92327 AAH19267 AAH02580
DBJ	BAG34867 BAA20369
PDB	1ZR9