BMRB Entry 6658

Assembly:

Entities:

Entity 1, FLIX3 - Fusion of ldb1-LID and Lhx3 LIM domains 182 residues - 20270.156 Da.

Residues 1-45 represent mouse ldb1 residues 295-339 (Swissprot P70662), residues 46-56 is a linker fusing the two proteins (GGSGGHMGSGG), where the HM is encoded by an NdeI restriction site; and residues 57-182 are from mouse Lhx3 residues 28-153 (Swissprot P50481)

Entity 2, ZINC ION_1 - Zn - 65.409 Da.

Samples:

sample_1: FLIX3 - Fusion of ldb1-LID and Lhx3 LIM domains, [U-99% 13C; U-99% 15N], 0.75 mM; ZINC ION 3 mM; DSS15 – 30 uM; DTT 1 mM; sodium phosphate 20 mM; sodium chloride 40 mM; D2O, [U-99% 2H], 10%; H2O 90%

sample_3: FLIX3 - Fusion of ldb1-LID and Lhx3 LIM domains 1.2 mM; ZINC ION 4.8 mM; DSS15 – 30 uM; DTT 1 mM; sodium phosphate 20 mM; sodium chloride 40 mM; H2O 90%; D2O, [U-99% 2H], 10%

sample_4: FLIX3 - Fusion of ldb1-LID and Lhx3 LIM domains, [U-10% 13C; U-99% 15N], 0.6 mM; ZINC ION 2.4 mM; DSS15 – 30 uM; DTT 1 mM; sodium phosphate 20 mM; sodium chloride 40 mM; D2O, [U-99% 2H], 90%; H2O 10%

sample_5: FLIX3 - Fusion of ldb1-LID and Lhx3 LIM domains, [U-99% 15N], 0.3 mM; ZINC ION 1.2 mM; DSS15 – 30 uM; DTT 1 mM; sodium phosphate 20 mM; sodium chloride 40 mM; D2O, [U-99% 2H], 90%; H2O 10%

sample_2: FLIX3 - Fusion of ldb1-LID and Lhx3 LIM domains, [U-99% 13C; U-99% 15N], 0.6 mM; ZINC ION 2.4 mM; DSS15 – 30 uM; DTT 1 mM; sodium phosphate 20 mM; sodium chloride 40 mM; D2O, [U-99% 2H], 99%; H2O 1%

sample_conditions_1: ionic strength: 60 mM; pH: 6.8; pressure: 1 atm; temperature: 310 K

Experiments:

Software:

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

ARIA v1.2, Linge, O'Donoghue and Nilges - chemical shift assignment, refinement

SPARKY, Goddard - data analysis, peak picking

TOPSPIN v1.2, Bruker Biospin - collection, processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

• Bruker DRX 600 MHz
• Bruker Avance 800 MHz