BMRB Entry 6516

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6516
MolProbity Validation Chart

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Title:
NMR assignment of the holo-acyl carrier protein (PfACP) from malaria parasite Plasmodium falciparum
Deposition date:
2005-02-19
Original release date:
2005-09-01
Authors:
Sharma, Alok; Sharma, Shailendra; Surolia, Namita; Sarma, Siddhartha
Citation:

Citation: Sharma, Alok; Sharma, Shailendra; Surolia, Namita; Sarma, Siddhartha. "NMR Assignment of the Holo-ACP from Malaria Parasite Plasmodium Falciparum"  J. Biomol. NMR 32, 260-260 (2005).
PubMed: 16132833

Assembly members:

Assembly members:
holo-acyl carrier protein from Plasmodium falciparum, polymer, 79 residues, 9006.2 Da.
PNS, non-polymer, 358.348 Da.

Natural source:

Natural source:   Common Name: Plasmodium falciparum   Taxonomy ID: 5833   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Plasmodium falciparum

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
holo-acyl carrier protein from Plasmodium falciparum: LKSTFDDIKKIISKQLSVEE DKIQMNSNFTKDLGADSLDL VELIMALEEKFNVTISDQDA LKINTVQDAIDYIEKNNKQ

Data sets:
Data typeCount
13C chemical shifts352
15N chemical shifts97
1H chemical shifts617
coupling constants79

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Polypeptide from Plasmodium falciparum, major conformer1
2Polypeptide from Plasmodium falciparum, minor conformer1
34'-phosphopantetheine prosthetic group, major conformer2
44'-phosphopantetheine prosthetic group, minor conformer2

Entities:

Entity 1, Polypeptide from Plasmodium falciparum, major conformer 79 residues - 9006.2 Da.

1   LEULYSSERTHRPHEASPASPILELYSLYS
2   ILEILESERLYSGLNLEUSERVALGLUGLU
3   ASPLYSILEGLNMETASNSERASNPHETHR
4   LYSASPLEUGLYALAASPSERLEUASPLEU
5   VALGLULEUILEMETALALEUGLUGLULYS
6   PHEASNVALTHRILESERASPGLNASPALA
7   LEULYSILEASNTHRVALGLNASPALAILE
8   ASPTYRILEGLULYSASNASNLYSGLN

Entity 2, 4'-phosphopantetheine prosthetic group, major conformer - C11 H23 N2 O7 P S - 358.348 Da.

1   PNS

Samples:

sample_1: holo-acyl carrier protein from Plasmodium falciparum, [U-98% 13C; U-98% 15N], 1.2 ± 0.2 mM; 4'-phosphopantetheine prosthetic group, [U-98% 13C; U-98% 15N], 1.2 ± 0.2 mM

conditions_1: pH: 6.5; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableconditions_1
1H-15N TOCSY-HSQCsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
H(CCO)NHsample_1not availableconditions_1
C(CCO)HNsample_1not availableconditions_1
HCCH_TOCSYsample_1not availableconditions_1
1H-13C HSQCsample_1not availableconditions_1
1H-15N 3D NOESYsample_1not availableconditions_1
1H-13C 3D NOESYsample_1not availableconditions_1

Software:

NMRPipe -

ANSIG -

NMR spectrometers:

  • Bruker Avance 700 MHz MHz

Related Database Links:

BMRB 16506 16529 16530 16531 16532 16533 16661
PDB
EMBL CDO62314
GB AAC63959 AAC71866 ETW20884 ETW27965 ETW39040
REF XP_001349595

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