BMRB Entry 6501

Name: Backbone 1H, 15N, 13Ca and sidechain 13Cb chemical shift assignments of SARS-CoV Nsp9
Published: 2005-03-17

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6501

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 15N, 13Ca and sidechain 13Cb chemical shift assignments of SARS-CoV Nsp9

Deposition date:

2005-02-12

Original release date:

2005-03-17

Authors:

Hu, Yunfei; Lescop, Ewen; Jin, Changwen

Citation:

Citation: Hu, Yunfei; Lescop, Ewen; Jin, Changwen. "Backbone dynamics of SARS-CoV Nsp9 by NMR spectroscopy" .

Assembly members:

Assembly members:
Nsp9, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source: Common Name: SARS-CoV Taxonomy ID: not available Superkingdom: Viruses Kingdom: Not applicable Genus/species: coronavirus Severe Acute Respiratory Syndrome associated coronavirus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Nsp9: NNELSPVALRQMSCAAGTTQ TACTDDNALAYYNNSKGGRF VLALLSDHQDLKWARFPKSD GTGTIYTELEPPCRFVTDTP KGPKVKYLYFIKGLNNLNRG MVLGSLAATVRLQ

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	189
15N chemical shifts	97
1H chemical shifts	97

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Nsp9, chain 1	1
2	Nsp9, chain 2	1

Entities:

Entity 1, Nsp9, chain 1 113 residues - Formula weight is not available

1

ASN

GLU

LEU

SER

PRO

VAL

ALA

LEU

ARG

2

GLN

MET

SER

CYS

ALA

GLY

THR

GLN

3

THR

ALA

CYS

THR

ASP

ASN

ALA

LEU

ALA

4

TYR

ASN

SER

LYS

GLY

ARG

PHE

5

VAL

LEU

ALA

LEU

SER

ASP

HIS

GLN

ASP

6

LEU

LYS

TRP

ALA

ARG

PHE

PRO

LYS

SER

ASP

7

GLY

THR

GLY

THR

ILE

TYR

THR

GLU

LEU

GLU

8

PRO

CYS

ARG

PHE

VAL

THR

ASP

THR

PRO

9

LYS

GLY

PRO

LYS

VAL

LYS

TYR

LEU

TYR

PHE

10

ILE

LYS

GLY

LEU

ASN

LEU

ASN

ARG

GLY

11

MET

VAL

LEU

GLY

SER

LEU

ALA

THR

VAL

12

ARG

LEU

GLN

Samples:

sample_1: Nsp9, chain 1, [U-90% 13C; U-90% 15N; U-80% 2H], 0.8 ± 0.2 mM; Nsp9, chain 2, [U-90% 13C; U-90% 15N; U-80% 2H], 0.8 ± 0.2 mM

conditions_1: pH: 6.8; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	sample_1	not available	conditions_1
HNCA	sample_1	not available	conditions_1
HN(CO)CA	sample_1	not available	conditions_1
HNCACB	sample_1	not available	conditions_1
HN(CO)CACB	sample_1	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Bruker Avance 500 MHz

PDB	1QZ8 1UW7 3EE7
DBJ	BAC81346 BAC81347 BAC81360 BAC81361 BAC81374
GB	AAP13439 AAP13442 AAP13566 AAP13575 AAP30028
REF	NP_828849 NP_828850 NP_828867 YP_003858583
SP	P0C6F5 P0C6F8 P0C6T7 P0C6U8 P0C6V9
AlphaFold	P0C6F5 P0C6F8 P0C6T7 P0C6U8 P0C6V9