BMRB Entry 6458

Name: Two-Dimensional 1H NMR Study of Human Ubiquitin
Published: 2006-12-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6458

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Two-Dimensional 1H NMR Study of Human Ubiquitin

Deposition date:

2005-01-13

Original release date:

2006-12-13

Authors:

Di Stefano, Deena; Wand, A.

Citation:

Citation: Di Stefano, Deena; Wand, A.. "Two-Dimensional 1H NMR Study of Human Ubiquitin: A Main Chain Directed Assignment and Structure Analysis" Biochemistry 26, 7272-7281 (1987).
PubMed: 2827748

Assembly members:

Assembly members:
Ubiquitin, polymer, 76 residues, 8579.94 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: Purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ubiquitin: MQIFVKTLTGKTITLEVEPS DTIENVKAKIQDKEGIPPDE QRLIFAGKQLQDGRTLSDYN IEKESTLHLVLRLRGG

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
1H chemical shifts	488

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ubiquitin	1

Entities:

Entity 1, Ubiquitin 76 residues - 8579.94 Da.

1

MET

GLN

ILE

PHE

VAL

LYS

THR

LEU

THR

GLY

2

LYS

THR

ILE

THR

LEU

GLU

VAL

GLU

PRO

SER

3

ASP

THR

ILE

GLU

ASN

VAL

LYS

ALA

LYS

ILE

4

GLN

ASP

LYS

GLU

GLY

ILE

PRO

ASP

GLU

5

GLN

ARG

LEU

ILE

PHE

ALA

GLY

LYS

GLN

LEU

6

GLN

ASP

GLY

ARG

THR

LEU

SER

ASP

TYR

ASN

7

ILE

GLU

LYS

GLU

SER

THR

LEU

HIS

LEU

VAL

8

LEU

ARG

LEU

ARG

GLY

Samples:

sample_1: Ubiquitin 8 mM; K3PO4 50 mM; H2O 90%; D2O 10%

sample_2: Ubiquitin 8 mM; K3PO4 50 mM; D2O 99.9%

conditions_1: pH: 5.8; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
DQF COSY	not available	not available	conditions_1
NOESY	not available	isotropic	conditions_1
TOCSY	not available	not available	conditions_1
RCT COSY	not available	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Bruker AM 500 500.13 MHz

EMBL	CAX72420
GB	ACH45547 EAW49110 EAX04506 EDX09116 EDX09117
REF	XP_002083531 XP_002083532 XP_004629745 XP_005502111 XP_005524281