BMRB Entry 6373

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6373

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Title:
1H, 13C and 15N backbone resonance assignments of LexA catalytic domain with the L89P/Q92W/D150H/E152A/K156A mutations
Deposition date:
2004-10-29
Original release date:
2005-06-03
Authors:
Okon, Mark; Pfuetzner, Richard; Vockovic, Marija; Little, John; Strynadka, Natalie; McIntosh, Lawrence
Citation:

Citation: Okon, Mark; Pfuetzner, Richard; Vockovic, Marija; Little, John; Strynadka, Natalie; McIntosh, Lawrence. "Letter to the Editor: Backbone chemical shift assignments of the LexA catalytic domain in its active conformation."  J. Biomol. NMR 31, 371-372 (2005).
PubMed: 15929009

Assembly members:

Assembly members:
catalytic domain of LexA L89P/Q92W/D150H/E152A/K156A mutant, polymer, 135 residues, 14850 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: Bacteria   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
catalytic domain of LexA L89P/Q92W/D150H/E152A/K156A mutant: LLQEEEEGLPLVGRVAAGEP LPAQWHIEGHYQVDPSLFKP NADFLLRVSGMSMKDIGIMD GDLLAVHKTQDVRNGQVVVA RIHDAVTVARLKKQGNKVEL LPENSEFKPIVVDLRQQSFT IEGLAVGVIRNGDWL

Data sets:
Data typeCount
13C chemical shifts377
15N chemical shifts125
1H chemical shifts125

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1catalytic domain of LexA L89P/Q92W/D150H/E152A/K156A mutant, chain 11
2catalytic domain of LexA L89P/Q92W/D150H/E152A/K156A mutant, chain 21

Entities:

Entity 1, catalytic domain of LexA L89P/Q92W/D150H/E152A/K156A mutant, chain 1 135 residues - 14850 Da.

1   LEULEUGLNGLUGLUGLUGLUGLYLEUPRO
2   LEUVALGLYARGVALALAALAGLYGLUPRO
3   LEUPROALAGLNTRPHISILEGLUGLYHIS
4   TYRGLNVALASPPROSERLEUPHELYSPRO
5   ASNALAASPPHELEULEUARGVALSERGLY
6   METSERMETLYSASPILEGLYILEMETASP
7   GLYASPLEULEUALAVALHISLYSTHRGLN
8   ASPVALARGASNGLYGLNVALVALVALALA
9   ARGILEHISASPALAVALTHRVALALAARG
10   LEULYSLYSGLNGLYASNLYSVALGLULEU
11   LEUPROGLUASNSERGLUPHELYSPROILE
12   VALVALASPLEUARGGLNGLNSERPHETHR
13   ILEGLUGLYLEUALAVALGLYVALILEARG
14   ASNGLYASPTRPLEU

Related Database Links:

PDB 1JHE 3JSO 3JSP 3K3R
GB KFH80209 KFH96244

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks