BMRB Entry 6372

Name: 1H and 15N Assigned Chemical Shifts for SARS N-Protein N-Terminate
Published: 2005-02-10

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6372

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H and 15N Assigned Chemical Shifts for SARS N-Protein N-Terminate

Deposition date:

2004-10-29

Original release date:

2005-02-10

Authors:

Zhong, Nan; Huang, Qing; Xia, Bin

Citation:

Citation: Zhong, Nan; Huang, Qing; Jin, Changwen; Xia, Bin. "Letter to the Editor: 1H, 13C and 15N resonance assignments of the N-terminal Domain of the SARS CoV Nucleocapsid Protein" J. Biomol. NMR 31, 79-80 (2005).

Assembly members:

Assembly members:
nucleocapsid protein, polymer, 179 residues, Formula weight is not available

Natural source:

Natural source: Common Name: SARS coronavirus Taxonomy ID: 227859 Superkingdom: Viruses Kingdom: not available Genus/species: Coronavirus SARS coronavirus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
nucleocapsid protein: MSDNGPQSNQRSAPRITFGG PTDSTDNNQNGGRNGARPKQ RRPQGLPNNTASWFTALTQH GKEELRFPRGQGVPINTNSG PDDQIGYYRRATRRVRGGDG KMKELSPRWYFYYLGTGPEA SLPYGANKEGIVWVATEGAL NTPKDHIGTRNPNNNAATVL QLPQGTTLPKGFYAEGSRG

Data sets:

assigned_chemical_shifts
- chemical_shift1

Data type	Count
1H chemical shifts	982
13C chemical shifts	628
15N chemical shifts	177

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SARS N-protein	1

Entities:

Entity 1, SARS N-protein 179 residues - Formula weight is not available

1

MET

SER

ASP

ASN

GLY

PRO

GLN

SER

ASN

GLN

2

ARG

SER

ALA

PRO

ARG

ILE

THR

PHE

GLY

3

PRO

THR

ASP

SER

THR

ASP

ASN

GLN

ASN

4

GLY

ARG

ASN

GLY

ALA

ARG

PRO

LYS

GLN

5

ARG

PRO

GLN

GLY

LEU

PRO

ASN

THR

6

ALA

SER

TRP

PHE

THR

ALA

LEU

THR

GLN

HIS

7

GLY

LYS

GLU

LEU

ARG

PHE

PRO

ARG

GLY

8

GLN

GLY

VAL

PRO

ILE

ASN

THR

ASN

SER

GLY

9

PRO

ASP

GLN

ILE

GLY

TYR

ARG

10

ALA

THR

ARG

VAL

ARG

GLY

ASP

GLY

11

LYS

MET

LYS

GLU

LEU

SER

PRO

ARG

TRP

TYR

12

PHE

TYR

LEU

GLY

THR

GLY

PRO

GLU

ALA

13

SER

LEU

PRO

TYR

GLY

ALA

ASN

LYS

GLU

GLY

14

ILE

VAL

TRP

VAL

ALA

THR

GLU

GLY

ALA

LEU

15

ASN

THR

PRO

LYS

ASP

HIS

ILE

GLY

THR

ARG

16

ASN

PRO

ASN

ALA

THR

VAL

LEU

17

GLN

LEU

PRO

GLN

GLY

THR

LEU

PRO

LYS

18

GLY

PHE

TYR

ALA

GLU

GLY

SER

ARG

GLY

Samples:

sample_1: nucleocapsid protein 1 mM

cond1: pH: 7; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	not available
HNCA	not available	not available	not available
HN(CO)CA	not available	not available	not available
HNCACB	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
HNCO	not available	not available	not available
HBHA(CO)NH	not available	not available	not available
HCCH-COSY	not available	not available	not available
HCCH-TOCSY	not available	not available	not available
CCH-TOCSY	not available	not available	not available
HNCA-D	not available	not available	not available
HN(CO)CA-D	not available	not available	not available
HNCACB-D	not available	not available	not available
HN(CO)CACB-D	not available	not available	not available

Software:

NMRView v5.0.4 -

NMR spectrometers:

BRUKER AVANCE 800 MHz
BRUKER AVANCE 600 MHz
BRUKER AVANCE 500 MHz

PDB	1SSK 2OFZ 2OG3
DBJ	BAC81358 BAC81372 BAC81386 BAC81400 BAC81414
GB	AAP13445 AAP13814 AAP30037 AAP30714 AAP33707
REF	NP_828858
SP	P59595 Q3I5I7
AlphaFold	P59595 Q3I5I7