BMRB Entry 6344

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6344
MolProbity Validation Chart

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Title:
1H, 13C, and 15N Chemical Shift Assignments for human protein HSPCO34
Deposition date:
2004-10-08
Original release date:
2010-08-25
Authors:
Ramelot, Theresa; Montelione, Guy; Acton, Thomas; Kennedy, Michael
Citation:

Citation: Ramelot, Theresa; Kennedy, Michael. "Solution NMR Structure of Human protein HSPCO34. Northeast Structural Genomics Target HR1958."  The BMRB entry is the only known published source for the data. (2004).

Assembly members:

Assembly members:
HR1958, polymer, 153 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: plasmid PET 14

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HR1958: MGHHHHHHSHMRKIDLCLSS EGSEVILATSSDEKHPPENI IDGNPETFWTTTGMFPQEFI ICFHKHVRIERLVIQSYFVQ TLKIEKSTSKEPVDFEQWIE KDLVHTEGQLQNEEIVAHGS ATYLRFIIVSAFDHFASVHS VSAEGTVVSNLSS

Data sets:
Data typeCount
1H chemical shifts1013
13C chemical shifts645
15N chemical shifts145

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HR19581

Entities:

Entity 1, HR1958 153 residues - Formula weight is not available

1   METGLYHISHISHISHISHISHISSERHIS
2   METARGLYSILEASPLEUCYSLEUSERSER
3   GLUGLYSERGLUVALILELEUALATHRSER
4   SERASPGLULYSHISPROPROGLUASNILE
5   ILEASPGLYASNPROGLUTHRPHETRPTHR
6   THRTHRGLYMETPHEPROGLNGLUPHEILE
7   ILECYSPHEHISLYSHISVALARGILEGLU
8   ARGLEUVALILEGLNSERTYRPHEVALGLN
9   THRLEULYSILEGLULYSSERTHRSERLYS
10   GLUPROVALASPPHEGLUGLNTRPILEGLU
11   LYSASPLEUVALHISTHRGLUGLYGLNLEU
12   GLNASNGLUGLUILEVALALAHISGLYSER
13   ALATHRTYRLEUARGPHEILEILEVALSER
14   ALAPHEASPHISPHEALASERVALHISSER
15   VALSERALAGLUGLYTHRVALVALSERASN
16   LEUSERSER

Samples:

sample_1: HR1958, [U-15N; U-13C], 1 mM; Mes 20 mM; NaCl 100 mM; CaCl2 5 mM; DTT 10 mM; NaN3 0.02%

sample_conditions: pH: 6.5; temperature: 298 K; ionic strength: 0.1 M

Experiments:

NameSampleSample stateSample conditions
Data collected on 600mHz Varian Inova spectrometer.sample_1not availablesample_conditions
[U-15N, U-13C]-HR1958sample_1not availablesample_conditions
2D 1H-15N HSQC - regular and NH2onlysample_1not availablesample_conditions
2D 1H-13C HSQC -aliph, alpha, aromsample_1not availablesample_conditions
3D HNCOsample_1not availablesample_conditions
3D HNCACBsample_1not availablesample_conditions
3D CBCA(CO)NHsample_1not availablesample_conditions
3D HCCH-COSYsample_1not availablesample_conditions
3D HCCH-TOCSYsample_1not availablesample_conditions
3D H(CC)(CO)NH-TOCSYsample_1not availablesample_conditions
3D (H)CC(CO)NH-TOCSYsample_1not availablesample_conditions
[U-15N, 5%-13C]-HR1958sample_1not availablesample_conditions
1H-13C HSQC to get stereospecific assignements for Leu, Val isopropyl groups.sample_1not availablesample_conditions
Data collected on 750mHz Varian Inova spectrometer.sample_1not availablesample_conditions
2D 1H-15N HSQCsample_1not availablesample_conditions
3D HNHAsample_1not availablesample_conditions
3D 15N-edited NOESY-HSQCsample_1not availablesample_conditions
3D 13C-edited NOESY-HSQC -aliph, aromsample_1not availablesample_conditions
4D CC-NOESY (2H2O)sample_1not availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 750 MHz
  • Varian Inova 800 MHz

Related Database Links:

PDB
GB AAH05245 AAQ03618 AAQ03994 ADQ32445 AIC56497
REF NP_001244683 NP_057210 XP_001149402 XP_002810844 XP_002810845
SP Q9Y547
AlphaFold Q9Y547

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks