BMRB Entry 6336

Name: Assignment of human AP4A hydrolase in complex with ATP
Published: 2005-11-14

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PDB ID: 1xsb
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6336
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assignment of human AP4A hydrolase in complex with ATP

Deposition date:

2004-10-05

Original release date:

2005-11-14

Authors:

Swarbrick, James

Citation:

Citation: Swarbrick, James; Buyya, S.; Gunawardana, D.; Fletcher, Jamie; Branson, Kim; Smith, Brian; Pepe, Salvatore; McLennan, A.; Gayler, K.; Gooley, Paul. "Letter to the Editor: 1H, 13C, and 15N resonance assignments of the 17 kDa Ap4A hydrolase from Homo sapiens in the presence and absence of ATP" J. Biomol. NMR 31, 181-182 (2005).

Assembly members:

Assembly members:
AP4A, polymer, 153 residues, Formula weight is not available
ATP, non-polymer, 507.181 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AP4A: GPLGSMALRACGLIIFRRCL IPKVDNNAIEFLLLQASDGI HHWTPPKGHVEPGEDDLETA LRATQEEAGIEAGQLTIIEG FKRELNYVARNKPKTVIYWL AEVKDYDVEIRLSHEHQAYR WLGLEEACQLAQFKEMKAAL QEGHQFLCSIEAL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts

Data type	Count
1H chemical shifts	1087
13C chemical shifts	652
15N chemical shifts	157

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AP4A hydrolase monomer	1
2	ATP	2

Entities:

Entity 1, AP4A hydrolase monomer 153 residues - Formula weight is not available

1

GLY

PRO

LEU

GLY

SER

MET

ALA

LEU

ARG

ALA

2

CYS

GLY

LEU

ILE

PHE

ARG

CYS

LEU

3

ILE

PRO

LYS

VAL

ASP

ASN

ALA

ILE

GLU

4

PHE

LEU

GLN

ALA

SER

ASP

GLY

ILE

5

HIS

TRP

THR

PRO

LYS

GLY

HIS

VAL

6

GLU

PRO

GLY

GLU

ASP

LEU

GLU

THR

ALA

7

LEU

ARG

ALA

THR

GLN

GLU

ALA

GLY

ILE

8

GLU

ALA

GLY

GLN

LEU

THR

ILE

GLU

GLY

9

PHE

LYS

ARG

GLU

LEU

ASN

TYR

VAL

ALA

ARG

10

ASN

LYS

PRO

LYS

THR

VAL

ILE

TYR

TRP

LEU

11

ALA

GLU

VAL

LYS

ASP

TYR

ASP

VAL

GLU

ILE

12

ARG

LEU

SER

HIS

GLU

HIS

GLN

ALA

TYR

ARG

13

TRP

LEU

GLY

LEU

GLU

ALA

CYS

GLN

LEU

14

ALA

GLN

PHE

LYS

GLU

MET

LYS

ALA

LEU

15

GLN

GLU

GLY

HIS

GLN

PHE

LEU

CYS

SER

ILE

16

GLU

ALA

LEU

Entity 2, ATP - C10 H16 N5 O13 P3 - 507.181 Da.

1

ATP

Samples:

sample_1: AP4A, [U-95% 13C; U-95% 15N], 1.0 mM; ADENOSINE-5'-TRIPHOSPHATE, [U-95% 13C; U-95% 15N], 1.5 mM

sample_conditions: pH: 6.5; temperature: 293 K; ionic strength: 40 mM

Experiments:

Name	Sample	Sample state	Sample conditions
not available	sample_1	not available	sample_conditions

Software:

nmrPipe v2.1, F. Delaglio -

XEASY v1.4, Kurt Wuthrich -

NMR spectrometers:

Varian Inova 600 MHz
Varian Inova 500 MHz

BMRB	6330
PDB	1XSA 1XSB 1XSC 3U53 4ICK 4IJX
GB	AAC50277 AAH04926 AAX36304 AAX36755 AAX41136
REF	NP_001152 NP_001231319 NP_671701 NP_671702 XP_001160435
SP	P50583
AlphaFold	P50583 P50583