BMRB Entry 6299

Name: Structure of a Chordin-like Cysteine-rich Repeat (VWC module) from Collagen IIA
Published: 2004-12-06

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PDB ID: 1u5m
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6299
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of a Chordin-like Cysteine-rich Repeat (VWC module) from Collagen IIA

Deposition date:

2004-08-26

Original release date:

2004-12-06

Authors:

O'Leary, J.; Hamilton, J.; Deane, C.; Valeyev, N.; Sandell, L.; Downing, A.

Citation:

Citation: O'Leary, J.; Hamilton, J.; Deane, C.; Valeyev, N.; Sandell, L.; Downing, A.. "Solution Structure and Dynamics of a Prototypical Chordin-like Cysteine-rich Repeat (von Willebrand Factor Type C Module) from Collagen IIA" J. Biol. Chem. 279, 53857-53866 (2004).
PubMed: 15466413

Assembly members:

Assembly members:
alpha 1 type II collagen isoform 1, polymer, 73 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Pichia pastoris Vector: PPICK9K-CRCOLIIA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
alpha 1 type II collagen isoform 1: YVEFQEAGSCVQDGQRYNDK DVWKPEPCRICVCDTGTVLC DDIICEDVKDCLSPEIPFGE CCPICPADLAAAA

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	177
15N chemical shifts	65
1H chemical shifts	414

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	alpha 1 type II collagen isoform 1	1

Entities:

Entity 1, alpha 1 type II collagen isoform 1 73 residues - Formula weight is not available

1

TYR

VAL

GLU

PHE

GLN

GLU

ALA

GLY

SER

CYS

2

VAL

GLN

ASP

GLY

GLN

ARG

TYR

ASN

ASP

LYS

3

ASP

VAL

TRP

LYS

PRO

GLU

PRO

CYS

ARG

ILE

4

CYS

VAL

CYS

ASP

THR

GLY

THR

VAL

LEU

CYS

5

ASP

ILE

CYS

GLU

ASP

VAL

LYS

ASP

6

CYS

LEU

SER

PRO

GLU

ILE

PRO

PHE

GLY

GLU

7

CYS

PRO

ILE

CYS

PRO

ALA

ASP

LEU

ALA

8

ALA

Samples:

sample_1: alpha 1 type II collagen isoform 1, [U-15N], 0.8 mM; phosphate buffer 50 mM; NaN3 0.02%; H2O 90%; D2O 10%

sample_2: alpha 1 type II collagen isoform 1, [U-15N], 0.8 mM; phosphate buffer 50 mM; NaN3 0.02%; D2O 99.9%

sample_3: alpha 1 type II collagen isoform 1, [U-13C; U-15N], 0.8 mM; phosphate buffer 50 mM; NaN3 0.02%; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	not available	not available	not available
HMQC-J	not available	not available	not available
2D NOESY	not available	not available	not available

Software:

CYANA v1.0.6 - refinement, structure solution

NMR spectrometers:

GE Omega 750 MHz