BMRB Entry 6271

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6271

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Title:
1H, 13C and 15N assignments of the ligand binding domain of LasR
Deposition date:
2004-07-23
Original release date:
2007-08-24
Authors:
Bottomley, Matthew; Muraglia, Ester; Bazzo, Renzo
Citation:

Citation: Bottomley, Matthew; Muraglia, E.; Bazzo, R.; Carf????, A.. "Molecular insights into quorum sensing in the human pathogen Pseudomonas aeruginosa from the structure of the virulence regulator LasR bound to its autoinducer"  J. Biol. Chem. 282, 13592-13600 (2007).
PubMed: 17363368

Assembly members:

Assembly members:
LasR Ligand binding domain, polymer, 173 residues, 20500 Da.
OHN, non-polymer, 297.390 Da.

Natural source:

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LasR Ligand binding domain: MALVDGFLELERSSGKLEWS AILQKMASDLGFSKILFGLL PKDSQDYENAFIVGNYPAAW REHYDRAGYARVDPTVSHCT QSVLPIFWEPSIYQTRKQHE FFEEASAAGLVYGLTMPLHG ARGELGALSLSVEAENRAEA NRFMESVLPTLWMLKDYALQ SGAGLAFEHPVSK

Data sets:
Data typeCount
13C chemical shifts499
15N chemical shifts159
1H chemical shifts159

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1LasR-LBD dimer1
23-oxo-c12-homoserine lactone2

Entities:

Entity 1, LasR-LBD dimer 173 residues - 20500 Da.

1   METALALEUVALASPGLYPHELEUGLULEU
2   GLUARGSERSERGLYLYSLEUGLUTRPSER
3   ALAILELEUGLNLYSMETALASERASPLEU
4   GLYPHESERLYSILELEUPHEGLYLEULEU
5   PROLYSASPSERGLNASPTYRGLUASNALA
6   PHEILEVALGLYASNTYRPROALAALATRP
7   ARGGLUHISTYRASPARGALAGLYTYRALA
8   ARGVALASPPROTHRVALSERHISCYSTHR
9   GLNSERVALLEUPROILEPHETRPGLUPRO
10   SERILETYRGLNTHRARGLYSGLNHISGLU
11   PHEPHEGLUGLUALASERALAALAGLYLEU
12   VALTYRGLYLEUTHRMETPROLEUHISGLY
13   ALAARGGLYGLULEUGLYALALEUSERLEU
14   SERVALGLUALAGLUASNARGALAGLUALA
15   ASNARGPHEMETGLUSERVALLEUPROTHR
16   LEUTRPMETLEULYSASPTYRALALEUGLN
17   SERGLYALAGLYLEUALAPHEGLUHISPRO
18   VALSERLYS

Entity 2, 3-oxo-c12-homoserine lactone - C16 H27 N O4 - 297.390 Da.

1   OHN

Related Database Links:

PDB 2UV0 3IX3 3IX4 3IX8 3JPU 4NG2
DBJ BAA06489 BAA06490 BAP24227 BAP51814 GAA16961
EMBL CAW28738 CCQ87458 CDH72122 CDH78244 CDI89583
GB AAA25874 AAG04819 AAT50467 ABJ10611 ABR86383
REF NP_250121 WP_003082999 WP_003100458 WP_003133239 WP_010792033
SP P25084
AlphaFold P25084

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks