BMRB Entry 6251

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6251

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Title:
Backbone Chemical Shift Assignments for V66W121 fragment of Staphylococcal Nuclease
Deposition date:
2004-06-24
Original release date:
2004-08-03
Authors:
Feng, Yingang; Liu, Dongsheng; Wang, Jinfeng
Citation:

Citation: Feng, Yingang; Liu, Dongsheng; Wang, Jinfeng. "Native-like partially folded conformations and folding process revealed in the N-terminal large fragments of staphylococcal nuclease: a study by NMR spectroscopy"  J. Mol. Biol. 330, 821-837 (2003).
PubMed: 12850150

Assembly members:

Assembly members:
V66W121, polymer, 121 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
V66W121: ATSTKKLHKEPATLIKAIDG DTVKLMYKGQPMTFRLLLVD TPETKHPKKGVEKYGPEASA FTKKMWENAKKIEVEFDKGQ RTDKYGRGLAYIYADGKMVN EALVRQGLAKVAYVYKPNNT H

Data sets:
Data typeCount
1H chemical shifts170
15N chemical shifts107
13C chemical shifts141

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1V66W1211

Entities:

Entity 1, V66W121 121 residues - Formula weight is not available

1   ALATHRSERTHRLYSLYSLEUHISLYSGLU
2   PROALATHRLEUILELYSALAILEASPGLY
3   ASPTHRVALLYSLEUMETTYRLYSGLYGLN
4   PROMETTHRPHEARGLEULEULEUVALASP
5   THRPROGLUTHRLYSHISPROLYSLYSGLY
6   VALGLULYSTYRGLYPROGLUALASERALA
7   PHETHRLYSLYSMETTRPGLUASNALALYS
8   LYSILEGLUVALGLUPHEASPLYSGLYGLN
9   ARGTHRASPLYSTYRGLYARGGLYLEUALA
10   TYRILETYRALAASPGLYLYSMETVALASN
11   GLUALALEUVALARGGLNGLYLEUALALYS
12   VALALATYRVALTYRLYSPROASNASNTHR
13   HIS

Samples:

sample_1: V66W121, [U-95% 13C; U-95% 15N], 1 mM; deuterated acetate, [U-99% 2H], 50 mM; DSS 50 uM; NaN3 0.02%

Ex-cond_1: pH: 5.0 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableEx-cond_1
HNCACBsample_1not availableEx-cond_1
CBCA(CO)NHsample_1not availableEx-cond_1
HBHA(CBCA)NHsample_1not availableEx-cond_1
HBHA(CBCA)(CO)NHsample_1not availableEx-cond_1
1H-15N slow exchange spectrasample_1not availableEx-cond_1

Software:

Felix v980 -

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 136 15357 1581 1582 16585 1704 17718 18013 1874 1875 1876 1877 1878 18788 188 189 2784 2785 4010 4052 4053 4905 494 495 496 497 530 5536 6250 644 6907 6908
PDB
DBJ BAB41979 BAB56977 BAB94634 BAF67032 BAF77694
EMBL CAA24594 CAG39855 CAG42530 CAI80436 CAQ49298
GB AAC14660 AAW36415 ABD22328 ABD29945 ABE02272
PRF 1109959A 710414A
REF NP_371339 NP_374001 NP_645586 WP_000141556 WP_000141557
SP P00644 Q5HHM4 Q6GB41 Q6GIK1 Q7A6P2
AlphaFold P00644 Q5HHM4 Q6GB41 Q6GIK1 Q7A6P2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks