BMRB Entry 6247
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PDB ID: 1th5
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6247
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Katoh, S.; Murata, K.; Kubota, Y.; Kumeta, H.; Ogura, K.; Inagaki, F.; Asayama, M.; Katoh, E.. "Refolding and purification of recombinant OsNifU1A domain II that was expressed by Escherichia coli." Protein Expr. Purif. 43, 149-156 (2005).
PubMed: 15978837
Assembly members:
NifU1, polymer, 74 residues, Formula weight is not available
Natural source: Common Name: rice Taxonomy ID: 4530 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Oryza sativa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PDEST32A
Entity Sequences (FASTA):
NifU1: MLELNEENVEKVLNEIRPYL
AGTGGGGLQFLMIKGPIVKV
RLTGPAAVVRTVRIAVSKKL
REKIPSIQIVQLLS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 344 |
| 15N chemical shifts | 80 |
| 1H chemical shifts | 547 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NifU1 | 1 |
Entities:
Entity 1, NifU1 74 residues - Formula weight is not available
| 1 | MET | LEU | GLU | LEU | ASN | GLU | GLU | ASN | VAL | GLU | ||||
| 2 | LYS | VAL | LEU | ASN | GLU | ILE | ARG | PRO | TYR | LEU | ||||
| 3 | ALA | GLY | THR | GLY | GLY | GLY | GLY | LEU | GLN | PHE | ||||
| 4 | LEU | MET | ILE | LYS | GLY | PRO | ILE | VAL | LYS | VAL | ||||
| 5 | ARG | LEU | THR | GLY | PRO | ALA | ALA | VAL | VAL | ARG | ||||
| 6 | THR | VAL | ARG | ILE | ALA | VAL | SER | LYS | LYS | LEU | ||||
| 7 | ARG | GLU | LYS | ILE | PRO | SER | ILE | GLN | ILE | VAL | ||||
| 8 | GLN | LEU | LEU | SER |
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