BMRB Entry 6173

Name: PfR48 final project
Published: 2006-02-26

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PDB ID: 2lp6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR6173
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

PfR48 final project

Deposition date:

2004-04-05

Original release date:

2006-02-26

Authors:

Snyder, David

Citation:

Citation: Snyder, David; Aramini, J.; Huang, Y.; Xiao, R.; Cort, J.; Shastry, R.; Ma, L.; Liu, J.; Rost, B.; Acton, T.; Kennedy, M.; Montelione, G.. "Solution Structure of the 50S Ribosomal Protein L35Ae from Pyrococcus furiosus: Northeast Strucutral Genomics Consortium target: Pfr48" .

Assembly members:

Assembly members:
PfR48, polymer, 95 residues, 9735.5 Da.

Natural source:

Natural source: Common Name: Pyrococcus furiosus Taxonomy ID: 2261 Superkingdom: Archaea Kingdom: not available Genus/species: Pyrococcus furiosus

Experimental source:

Experimental source: Production method: recombinant technology Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PfR48: MRIKGVVLSYRRSKENQHNN VMIIKPLDVNSREEASKLIG RLVLWKSPSGKILKGKIVRV HGTKGAVRARFEKGLPGQAL GDYVEIVLEHHHHHH

Data sets:

assigned_chemical_shifts
- Assigned_chemical_shift_list_1
- Assigned_chemical_shift_list_2
coupling_constants
- JNH_1

Data type	Count
13C chemical shifts	396
15N chemical shifts	93
1H chemical shifts	656
coupling constants	42

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PfR48	1

Entities:

Entity 1, PfR48 95 residues - 9735.5 Da.

1

MET

ARG

ILE

LYS

GLY

VAL

LEU

SER

TYR

2

ARG

SER

LYS

GLU

ASN

GLN

HIS

ASN

3

VAL

MET

ILE

LYS

PRO

LEU

ASP

VAL

ASN

4

SER

ARG

GLU

ALA

SER

LYS

LEU

ILE

GLY

5

ARG

LEU

VAL

LEU

TRP

LYS

SER

PRO

SER

GLY

6

LYS

ILE

LEU

LYS

GLY

LYS

ILE

VAL

ARG

VAL

7

HIS

GLY

THR

LYS

GLY

ALA

VAL

ARG

ALA

ARG

8

PHE

GLU

LYS

GLY

LEU

PRO

GLY

GLN

ALA

LEU

9

GLY

ASP

TYR

VAL

GLU

ILE

VAL

LEU

GLU

HIS

10

HIS

Samples:

sample_PfR48.NC.RX.7.85: PfR48, [U-15N], 1.07 mM; NaCl 100 mM; CaCl2 5 mM

sample_PfR48.NC5.LYD.III.3-6.5: PfR48, [U-15N], 0.29 mM; NaCl 100 mM; CaCl2 5 mM

sample_PfR48.PfR48-21.1-N-GF-6.5: PfR48, [U-15N], 0.5 mM; NaCl 100 mM; CaCl2 5 mM

Sample_condition_list_1: ionic strength: 0.1 M; pH: 6.5; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
HNCACB	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
HNCO	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
NH_HSQC	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
NH_HSQC	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
HNcoCA	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
HNcoCACB	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
HAcacoNH	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
HAcaNH	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
HNCACB (tau c)	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
HNHA	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
CCONH	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
HCCH-COSY	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
HCCH-TOCSY	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
hetNOE (t0)	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
hetNOE (t3)	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
CH_HSQC (aliph)	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
CH_HSQC (aliph)	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
hbCBcgcdceHE	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
HBCBcgcdHD	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
MUSIC_gly_CA	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
MUSIC_gly_CO	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
CH_HSQC (arom)	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
15N-edited_NOESY	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
13C_edited_NOESY (aliph)	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
13C_edited_NOESY (D2O aliph)	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
13C_edited_NOESY (arom)	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
HCCcoNH-TOCSY	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
4D-C13-NOESY	sample_PfR48.NC.RX.7.85	not available	Sample_condition_list_1
NH_HSQC (larger SW1)	sample_PfR48.NC5.LYD.III.3-6.5	not available	Sample_condition_list_1
CH_HSQC	sample_PfR48.NC5.LYD.III.3-6.5	not available	Sample_condition_list_1
NH_HSQC (15 min)	sample_PfR48.PfR48-21.1-N-GF-6.5	not available	Sample_condition_list_1

Software:

VNMR_6.1C - data collection

NMRPipe - data processing

SPARKY - spectral visualization

AutoAssign -

AutoStruct - automated structural determination

SPINS - integrated spectral analysis software

NMR spectrometers:

varian Baker 600 MHz
varian UNITY Inova 500 MHz
Varian INOVA 750 MHz
varian INOVA 800 MHz
varian UNITY Inova 600 MHz

PDB	1SQR 2LP6 3J21
GB	AAL81996 AFN04768
REF	NP_579601 WP_011013011 YP_006493060
SP	P20299 Q8TZV6
AlphaFold	P20299 Q8TZV6