BMRB Entry 6092

Title:
1H, 13C, and 15N Chemical Shift Assignments for a complex of PDZ2 from PTP-BL with the C-terminus of RIL (reversion induced LIM)
Deposition date:
2004-02-04
Original release date:
2011-08-11
Authors:
Walma, Tine; Vuister, Geerten
Citation:

Citation: Walma, Tine; Aelen, J.; Nabuurs, S.; Oostendorp, M.; van den Berk, L.; Hendriks, W.; Vuister, Geerten. "A closed binding pocket and global destabilization modify the binding properties of an alternatively spliced form of the second PDZ domain of PTP-BL"  Structure 12, 11-20 (2004).
PubMed: 14725761

Assembly members:

Assembly members:
Second PDZ domain from PTP-BL, polymer, 102 residues, 9752.07 Da.
C-terminus from RIL, polymer, 12 residues, 1301.55 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts278
1H chemical shifts622
15N chemical shifts105
heteronuclear NOE values82
T1 relaxation values82
T2 relaxation values82

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ2 from PTP-BL1
2RIL C-terminus2

Entities:

Entity 1, PDZ2 from PTP-BL 102 residues - 9752.07 Da.

1   METHISHISHISHISHISHISMETLYSPRO
2   GLYASPTHRPHEGLUVALGLULEUALALYS
3   THRASPGLYSERLEUGLYILESERVALTHR
4   GLYGLYVALASNTHRSERVALARGHISGLY
5   GLYILETYRVALLYSALAILEILEPROLYS
6   GLYALAALAGLUSERASPGLYARGILEHIS
7   LYSGLYASPARGVALLEUALAVALASNGLY
8   VALSERLEUGLUGLYALATHRHISLYSGLN
9   ALAVALGLUTHRLEUARGASNTHRGLYGLN
10   VALVALHISLEULEULEUGLULYSGLYGLN
11   VALPRO

Entity 2, RIL C-terminus 12 residues - 1301.55 Da.

1   VALALAVALTYRPROASNALALYSVALGLU
2   LEUVAL

Samples:

sample_1: Second PDZ domain from PTP-BL, [U-15N; U-13C], 1 mM; C-terminus from RIL 3.55 mM; K2HPO4/KH2PO4 50 mM; KCl 50 mM

conditions_1: pH: 6.8; temperature: 298 K; ionic strength: 0.1 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions

Software:

NMRPipe v1.8 - spectral processing

XEASY v1.2 - peak integration, assignment

XPLOR v3.851 - simulated annealing, restrained MD refinement in water

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

GenBank AAC42056 AAC42055
PDB
BMRB 6091 6060 5131
AlphaFold Q64499

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks