BMRB Entry 6088

Title:
E6-bind Trp-cage (E6apn1)
Deposition date:
2004-01-29
Original release date:
2008-07-17
Authors:
Liu, Y.; Liu, Z.; Androphy, E.; Chen, J.; Baleja, J.
Citation:

Citation: Liu, Y.; Liu, Z.; Androphy, E.; Chen, J.; Baleja, J.. "DESIGN AND CHARACTERIZATION OF HELICAL PEPTIDES THAT BIND THE E6 PROTEIN OF PAPILLOMAVIRUS"  Biochemistry 43, 7421-7431 (2004).
PubMed: 15182185

Assembly members:

Assembly members:
E6apn1, polymer, 25 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Papillomavirus   Taxonomy ID: 10558   Superkingdom: Viruses   Kingdom: not available   Genus/species: Papillomavirus not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
E6apn1: XALQELLGQWLKDGGPSSGR PPPSX

Data sets:
Data typeCount
1H chemical shifts149

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1E6apn1 peptide1

Entities:

Entity 1, E6apn1 peptide 25 residues - Formula weight is not available

1   ACEALALEUGLNGLULEULEUGLYGLNTRP
2   LEULYSASPGLYGLYPROSERSERGLYARG
3   PROPROPROSERNH2

Samples:

sample_1: E6apn15.0 – 6.0 mM; imidazole, [U-2H], 10 mM; ZnSO4 4 mM; DTT 1 mM; H2O 90%; D2O 10%

sample_2: E6apn15.0 – 6.0 mM; imidazole, [U-2H], 10 mM; ZnSO4 4 mM; DTT 1 mM; H2O 90%; D2O 99.96%

sample_cond_1: pH: 6.5; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYnot availablenot availablesample_cond_1
2D NOESYnot availablenot availablesample_cond_1

Software:

xwinnmr v3.5 - collection, processing

CNS v1.1 - refinement, structure solution

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

BMRB 15169 5292
PDB