BMRB Entry 6062

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PDB ID: 1s2f
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6062
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assignments for the Negative Regulator of Splicing from Rous Sarcoma Virus residues 907 to 929

Deposition date:

2004-01-06

Original release date:

2004-10-29

Authors:

Cabello-Villegas, Javier; Guiles, Keith; Soto, Ana; Yu, Ping; Beemon, Karen; Wang, Yun-Xing

Citation:

Citation: Cabello-Villegas, Javier; Giles, Keith; Soto, Ana; Yu, Ping; Mougin, A.; Beemon, Karen; Wang, Yun-Xing. "Solution structure of the pseudo-5' splice site of a retroviral splicing suppressor" RNA 10, 1388-1398 (2004).
PubMed: 15317975

Assembly members:

Assembly members:
RNA stem-loop, polymer, 23 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Rous sarcoma virus Taxonomy ID: 11886 Superkingdom: Viruses Kingdom: not available Genus/species: alpharetrovirus Rous sarcoma virus

Experimental source:

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA stem-loop: GGGGAGUGGUUUGUAUCCUU CCC

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	153
15N chemical shifts	15
31P chemical shifts	17

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NRS23	1

Entities:

Entity 1, NRS23 23 residues - Formula weight is not available

1

G

A

G

U

G

U

2

U

G

U

A

U

C

U

3

C

Samples:

Sample_1: RNA stem-loop, [U-13C; U-15N], 1.2 mM; sodium phosphate buffer 10 mM; NaCl 25 mM

condition_1: pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-13C NOESY-HSQC	not available	not available	not available
2D NOESY	not available	not available	not available
15N/1H HMQC	not available	not available	not available
HCCH-TOCSY	not available	not available	not available
HCCH-COSY	not available	not available	not available
HNCCCH	not available	not available	not available
HCP	not available	not available	not available

Software:

NMRDraw - Spectra analysis

NMRView - Spectra analysis

NMRPipe - Spectra processing

NMR spectrometers:

Varian INOVA 800 MHz
Varian INOVA 600 MHz
Varian INOVA 500 MHz
Bruker DRX 500 MHz