BMRB Entry 6052

Click here to enlarge.

PDB ID: 1sgo
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6052
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone and side chain 1H, 13C, and 15N chemical shift assignments for Haemophilus human protein HR969

Deposition date:

2003-12-16

Original release date:

2004-09-30

Authors:

Ramelot, Theresa; Montelione, Gaetano; Kennedy, Michael

Citation:

Citation: Ramelot, Theresa; Montelione, Gaetano; Kennedy, Michael. "Backbone and side chain 1H, 13C, and 15N chemical shift assignments for Haemophilus human protein HR969" .

Assembly members:

Assembly members:
HR969, polymer, 149 residues, 16800 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HR969: MGHHHHHHSHMETDCNPMEL SSMSGFEEGSELNGFEGTDM KDMRLEAEAVVNDVLFAVNN MFVSKSLRCADDVAYINVET KERNRYCLELTEAGLKVVGY AFDQVDDHLQTPYHETVYSL LDTLSPAYREAFGNALLQRL EALKRDGQS

Data sets:

assigned_chemical_shifts
- HR969_shift

Data type	Count
1H chemical shifts	926
13C chemical shifts	583
15N chemical shifts	143

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HR969	1

Entities:

Entity 1, HR969 149 residues - 16800 Da.

1

MET

GLY

HIS

SER

HIS

2

MET

GLU

THR

ASP

CYS

ASN

PRO

MET

GLU

LEU

3

SER

MET

SER

GLY

PHE

GLU

GLY

SER

4

GLU

LEU

ASN

GLY

PHE

GLU

GLY

THR

ASP

MET

5

LYS

ASP

MET

ARG

LEU

GLU

ALA

GLU

ALA

VAL

6

VAL

ASN

ASP

VAL

LEU

PHE

ALA

VAL

ASN

7

MET

PHE

VAL

SER

LYS

SER

LEU

ARG

CYS

ALA

8

ASP

VAL

ALA

TYR

ILE

ASN

VAL

GLU

THR

9

LYS

GLU

ARG

ASN

ARG

TYR

CYS

LEU

GLU

LEU

10

THR

GLU

ALA

GLY

LEU

LYS

VAL

GLY

TYR

11

ALA

PHE

ASP

GLN

VAL

ASP

HIS

LEU

GLN

12

THR

PRO

TYR

HIS

GLU

THR

VAL

TYR

SER

LEU

13

LEU

ASP

THR

LEU

SER

PRO

ALA

TYR

ARG

GLU

14

ALA

PHE

GLY

ASN

ALA

LEU

GLN

ARG

LEU

15

GLU

ALA

LEU

LYS

ARG

ASP

GLY

GLN

SER

Samples:

sample_1: HR969, [U-15N; U-13C], 1 mM; MES 20 mM; NaCl 100 mM; CaCl2 5 mM

sample_2: HR969, [U-15N], 1 mM; MES 20 mM; NaCl 100 mM; CaCl2 5 mM

sample_3: HR969, [U-5% 13C; U-100% 15N], 1 mM; MES 20 mM; NaCl 100 mM; CaCl2 5 mM

cond_1: pH: 6.5; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	cond_1
1H-13C HSQC (aliph)	not available	not available	cond_1
15N-edited NOESY-HQSC	not available	not available	cond_1
HCC-TOCSY-NNH	not available	not available	cond_1
HCCH-TOCSY	not available	not available	cond_1
13C-edited-NOESY-HSQC (aliph)	not available	not available	cond_1
CBCACOCAHA	not available	not available	cond_1
HNCA	not available	not available	cond_1
HN(CO)CA	not available	not available	cond_1
HNCO	not available	not available	cond_1
HNCACB	not available	not available	cond_1
CBCA(CO)NH	not available	not available	cond_1
CC-TOCSY-NNH	not available	not available	cond_1
HNHA	not available	not available	cond_1
4D CC-NOESY	not available	not available	cond_1
1H-13C HSQC (arom)	not available	not available	cond_1
1H-13C HSQC (alpha)	not available	not available	cond_1
13C-edited-NOESY-HSQC (arom)	not available	not available	cond_1

Software:

Felix v98 - processing data

Sparky v3.98 - analyzing, peak picking spectra

Autostructure v2 - assigning noe peaks

XPLOR NIH v2.0.6 - structure calculations

NMR spectrometers:

Varian Inova 500 MHz
Varian Inova 600 MHz
Varian Inova 750 MHz
Varian Inova 800 MHz

PDB	1SGO
DBJ	BAA91380 BAE00760 BAG52902
GB	AAF36130 AAH04818 AIC51518 EAW81634 EAW81635
REF	NP_001181120 NP_001229499 NP_001258833 NP_001258834 NP_001258835
SP	Q4R812 Q9P0R6
AlphaFold	Q4R812 Q9P0R6