BMRB Entry 5935
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5935
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Zangger, Klaus; Pervushin, Konstantin; Sterk, Heinz; Lange, Alex; Okar, David. "Sequence-specific 1H, 13C and 15N resonance assignments of rat liver
fructose-2,6-bisphosphatase domain " J. Biomol. NMR 27, 281-282 (2003).
Assembly members:
Fructose-2,6-bisphosphatase, polymer, 221 residues, 25554 Da.
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 163 |
| 13C chemical shifts | 558 |
| 15N chemical shifts | 163 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FBP-2 monomer | 1 |
Entities:
Entity 1, FBP-2 monomer 221 residues - 25554 Da.
| 1 | MET | ARG | SER | ILE | TYR | LEU | CYS | ARG | HIS | GLY | ||||
| 2 | GLU | SER | GLU | LEU | ASN | LEU | ARG | GLY | ARG | ILE | ||||
| 3 | GLY | GLY | ASP | SER | GLY | LEU | SER | ALA | ARG | GLY | ||||
| 4 | LYS | GLN | TYR | ALA | TYR | ALA | LEU | ALA | ASN | PHE | ||||
| 5 | ILE | ARG | SER | GLN | GLY | ILE | SER | SER | LEU | LYS | ||||
| 6 | VAL | TRP | THR | SER | HIS | MET | LYS | ARG | THR | ILE | ||||
| 7 | GLN | THR | ALA | GLU | ALA | LEU | GLY | VAL | PRO | TYR | ||||
| 8 | GLU | GLN | TRP | LYS | ALA | LEU | ASN | GLU | ILE | ASP | ||||
| 9 | ALA | GLY | VAL | CYS | GLU | GLU | MET | THR | TYR | GLU | ||||
| 10 | GLU | ILE | GLN | GLU | HIS | TYR | PRO | GLU | GLU | PHE | ||||
| 11 | ALA | LEU | ARG | ASP | GLN | ASP | LYS | TYR | ARG | TYR | ||||
| 12 | ARG | TYR | PRO | LYS | GLY | GLU | SER | TYR | GLU | ASP | ||||
| 13 | LEU | VAL | GLN | ARG | LEU | GLU | PRO | VAL | ILE | MET | ||||
| 14 | GLU | LEU | GLU | ARG | GLN | GLU | ASN | VAL | LEU | VAL | ||||
| 15 | ILE | CYS | HIS | GLN | ALA | VAL | MET | ARG | CYS | LEU | ||||
| 16 | LEU | ALA | TYR | PHE | LEU | ASP | LYS | SER | SER | ASP | ||||
| 17 | GLU | LEU | PRO | TYR | LEU | LYS | CYS | PRO | LEU | HIS | ||||
| 18 | THR | VAL | LEU | LYS | LEU | THR | PRO | VAL | ALA | TYR | ||||
| 19 | GLY | CYS | ARG | VAL | GLU | SER | ILE | TYR | LEU | ASN | ||||
| 20 | VAL | GLU | ALA | VAL | ASN | THR | HIS | ARG | ASP | LYS | ||||
| 21 | PRO | GLU | ASN | VAL | ASP | ILE | THR | ARG | GLU | ALA | ||||
| 22 | GLU | GLU | ALA | LEU | ASP | THR | VAL | PRO | ALA | HIS | ||||
| 23 | TYR |
Samples:
sample_1: Fructose-2,6-bisphosphatase, [U-95% 13C; U-95 15N], 2.7 mM; sodium phosphate 100 mM
sample_2: Fructose-2,6-bisphosphatase 1.5 mM; sodium phosphate 100 mM
Ex-cond_1: pH: 7.0; temperature: 298 K; ionic strength: 1.2 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| TROSY versions used for all experiments; | not available | not available | Ex-cond_1 |
| 2H decoupling used on triply-labeled sample. | not available | not available | Ex-cond_1 |
Software:
NMRPipe v03/2002 - processing
NMRView v5.0.3 - analysis
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian INOVA 600 MHz
- Bruker Avance 800 MHz
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAE21271 BAG36785 BAG62397 |
| EMBL | CAA33606 CAA33607 CAA68694 |
| GB | AAA30696 AAA40624 AAA79008 AAB19845 AAH96077 |
| PIR | S77704 |
| REF | NP_001257992 NP_001257993 NP_001258733 NP_001258734 NP_002616 |
| SP | P07953 P16118 P49872 P70266 |
| TPG | DAA12796 |
| AlphaFold | P07953 P16118 P49872 P70266 P07953 P16118 P49872 P70266 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks