BMRB Entry 5770
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PDB ID: 1nwd
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5770
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Yap, Kyoko; Yuan, Tao; Mal, Tapas; Vogel, Hans; Ikura, Mitsu. "Structural basis for simultaneous binding of two carboxy-terminal peptides of
plant glutamate decarboxylase to calmodulin " J. Mol. Biol. 328, 193-204 (2003).
PubMed: 12684008
Assembly members:
Calmodulin, polymer, 148 residues, Formula weight is not available
Glutamate decarboxylase, polymer, 28 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.
Natural source: Common Name: African clawed frog Taxonomy ID: 8355 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Xenopus laevis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pAS
Entity Sequences (FASTA):
Calmodulin: ADQLTEEQIAEFKEAFSLFD
KDGDGTITTKELGTVMRSLG
QNPTEAELQDMINEVDADGN
GTIDFPEFLTMMARKMKDTD
SEEEIREAFRVFDKDGNGYI
SAAELRHVMTNLGEKLTDEE
VDEMIREADIDGDGQVNYEE
FVQMMTAK
Glutamate decarboxylase: GSHKKTDSEVQLEMITAWKK
FVEEKKKK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 1437 |
| 13C chemical shifts | 908 |
| 15N chemical shifts | 210 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | calmodulin | 1 |
| 2 | glutamate decarboxylase peptide A | 2 |
| 3 | glutamate decarboxylase peptide B | 2 |
| 4 | calcium ion I | 3 |
| 5 | calcium ion II | 3 |
| 6 | calcium ion III | 3 |
| 7 | calcium ion IV | 3 |
Entities:
Entity 1, calmodulin 148 residues - Formula weight is not available
| 1 | ALA | ASP | GLN | LEU | THR | GLU | GLU | GLN | ILE | ALA | ||||
| 2 | GLU | PHE | LYS | GLU | ALA | PHE | SER | LEU | PHE | ASP | ||||
| 3 | LYS | ASP | GLY | ASP | GLY | THR | ILE | THR | THR | LYS | ||||
| 4 | GLU | LEU | GLY | THR | VAL | MET | ARG | SER | LEU | GLY | ||||
| 5 | GLN | ASN | PRO | THR | GLU | ALA | GLU | LEU | GLN | ASP | ||||
| 6 | MET | ILE | ASN | GLU | VAL | ASP | ALA | ASP | GLY | ASN | ||||
| 7 | GLY | THR | ILE | ASP | PHE | PRO | GLU | PHE | LEU | THR | ||||
| 8 | MET | MET | ALA | ARG | LYS | MET | LYS | ASP | THR | ASP | ||||
| 9 | SER | GLU | GLU | GLU | ILE | ARG | GLU | ALA | PHE | ARG | ||||
| 10 | VAL | PHE | ASP | LYS | ASP | GLY | ASN | GLY | TYR | ILE | ||||
| 11 | SER | ALA | ALA | GLU | LEU | ARG | HIS | VAL | MET | THR | ||||
| 12 | ASN | LEU | GLY | GLU | LYS | LEU | THR | ASP | GLU | GLU | ||||
| 13 | VAL | ASP | GLU | MET | ILE | ARG | GLU | ALA | ASP | ILE | ||||
| 14 | ASP | GLY | ASP | GLY | GLN | VAL | ASN | TYR | GLU | GLU | ||||
| 15 | PHE | VAL | GLN | MET | MET | THR | ALA | LYS |
Entity 2, glutamate decarboxylase peptide A 28 residues - Formula weight is not available
| 1 | GLY | SER | HIS | LYS | LYS | THR | ASP | SER | GLU | VAL | ||||
| 2 | GLN | LEU | GLU | MET | ILE | THR | ALA | TRP | LYS | LYS | ||||
| 3 | PHE | VAL | GLU | GLU | LYS | LYS | LYS | LYS |
Entity 3, calcium ion I - Ca - 40.078 Da.
| 1 | CA |
Related Database Links:
| PDB | 1NWD |
| GB | AAA33709 AAA33710 |
| SP | Q07346 |
| GenBank | AAA33710 AAA33709 |
| SWISS-PROT | Q07346 |
| AlphaFold | Q07346 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks