BMRB Entry 5759

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5759

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Title:
A model for effector activity in a highly specific biological electron transfer complex: The cytochrome P450cam-putidaredoxin couple
Deposition date:
2003-03-31
Original release date:
2004-04-07
Authors:
Pochapsky, Susan; Pochapsky, Thomas; Wei, Julie
Citation:

Citation: Pochapsky, Susan; Pochapsky, Thomas; Wei, Julie. "A Model for Effector Activity in a Highly Specific Biological Electron Transfer Complex: The Cytochrome P450cam-Putidaredoxin Couple"  Biochemistry 42, 5649-5656 (2003).
PubMed: 12741821

Assembly members:

Assembly members:
cytochrome P450cam, polymer, 414 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.

Natural source:

Natural source:   Common Name: Pseudomonas putida   Taxonomy ID: 303   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Pseudomonas putida

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cytochrome P450cam: TTETIQSNANLAPLPPHVPE HLVFDFDMYNPSNLSAGVQE AWAVLQESNVPDLVWTRCNG GHWIATRGQLIREAYEDYRH FSSECPFIPREAGEAYDFIP TSMDPPEQRQFRALANQVVG MPVVDKLENRIQELACSLIE SLRPQGQCNFTEDYAEPFPI RIFMLLAGLPEEDIPHLKYL TDQMTRPDGSMTFAEAKEAL YDYLIPIIEQRRQKPGTDAI SIVANGQVNGRPITSDEAKR MCGLLLVGGLDTVVNFLSFS MEFLAKSPEHRQELIERPER IPAACEELLRRFSLVADGRI LTSDYEFHGVQLKKGDQILL PQMLSGLDERENAAPMHVDF SRQKVSHTTFGHGSHLCLGQ HLARREIIVTLKEWLTRIPD FSIAPGAQIQHKSGIVSGVQ ALPLVWDPATTKAV

Data sets:
Data typeCount
13C chemical shifts404
1H chemical shifts143
15N chemical shifts143

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1cytochrome P450cam1
2Heme2

Entities:

Entity 1, cytochrome P450cam 414 residues - Formula weight is not available

1   THRTHRGLUTHRILEGLNSERASNALAASN
2   LEUALAPROLEUPROPROHISVALPROGLU
3   HISLEUVALPHEASPPHEASPMETTYRASN
4   PROSERASNLEUSERALAGLYVALGLNGLU
5   ALATRPALAVALLEUGLNGLUSERASNVAL
6   PROASPLEUVALTRPTHRARGCYSASNGLY
7   GLYHISTRPILEALATHRARGGLYGLNLEU
8   ILEARGGLUALATYRGLUASPTYRARGHIS
9   PHESERSERGLUCYSPROPHEILEPROARG
10   GLUALAGLYGLUALATYRASPPHEILEPRO
11   THRSERMETASPPROPROGLUGLNARGGLN
12   PHEARGALALEUALAASNGLNVALVALGLY
13   METPROVALVALASPLYSLEUGLUASNARG
14   ILEGLNGLULEUALACYSSERLEUILEGLU
15   SERLEUARGPROGLNGLYGLNCYSASNPHE
16   THRGLUASPTYRALAGLUPROPHEPROILE
17   ARGILEPHEMETLEULEUALAGLYLEUPRO
18   GLUGLUASPILEPROHISLEULYSTYRLEU
19   THRASPGLNMETTHRARGPROASPGLYSER
20   METTHRPHEALAGLUALALYSGLUALALEU
21   TYRASPTYRLEUILEPROILEILEGLUGLN
22   ARGARGGLNLYSPROGLYTHRASPALAILE
23   SERILEVALALAASNGLYGLNVALASNGLY
24   ARGPROILETHRSERASPGLUALALYSARG
25   METCYSGLYLEULEULEUVALGLYGLYLEU
26   ASPTHRVALVALASNPHELEUSERPHESER
27   METGLUPHELEUALALYSSERPROGLUHIS
28   ARGGLNGLULEUILEGLUARGPROGLUARG
29   ILEPROALAALACYSGLUGLULEULEUARG
30   ARGPHESERLEUVALALAASPGLYARGILE
31   LEUTHRSERASPTYRGLUPHEHISGLYVAL
32   GLNLEULYSLYSGLYASPGLNILELEULEU
33   PROGLNMETLEUSERGLYLEUASPGLUARG
34   GLUASNALAALAPROMETHISVALASPPHE
35   SERARGGLNLYSVALSERHISTHRTHRPHE
36   GLYHISGLYSERHISLEUCYSLEUGLYGLN
37   HISLEUALAARGARGGLUILEILEVALTHR
38   LEULYSGLUTRPLEUTHRARGILEPROASP
39   PHESERILEALAPROGLYALAGLNILEGLN
40   HISLYSSERGLYILEVALSERGLYVALGLN
41   ALALEUPROLEUVALTRPASPPROALATHR
42   THRLYSALAVAL

Entity 2, Heme - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Related Database Links:

BMRB 16753 17415 19038
PDB 1AKD 1C8J 1CP4 1DZ4 1DZ6 1DZ8 1DZ9 1GEB 1GEK 1GEM 1GJM 1IWI 1IWJ 1IWK 1J51 1K2O 1LWL 1MPW 1NOO 1O76 1P2Y 1P7R 1PHA 1PHB 1PHC 1PHD 1PHE 1PHF 1PHG 1QMQ 1RE9 1RF9 1T85 1T86 1T87 1T88 1UYU 1YRC 1YRD 2A1M 2A1N 2A1O 2CP4 2CPP 2FE6 2FER 2FEU 2FRZ 2GQX 2GR6 2H7Q 2H7R 2H7S 2L8M 2LQD 2M56 2QBL 2QBM 2QBN 2QBO 2Z97 2ZAW 2ZAX 2ZUH 2ZUI 2ZUJ 2ZWT 2ZWU 3CP4 3CPP 3FWF 3FWG 3FWI 3FWJ 3L61 3L62 3L63 3OIA 3OL5 3P6M 3P6N 3P6O 3P6P 3P6Q 3P6R 3P6S 3P6T 3P6U 3P6V 3P6W 3P6X 3W9C 4CP4 4CPP 4EK1 4G3R 4JWS 4JWU 4JX1 4KKY 4L49 4L4A 4L4B 4L4C 4L4D 4L4E 4L4F 4L4G 5CP4 5CPP 6CP4 6CPP 7CPP 8CPP
DBJ BAN13286
GB AAA25760
REF WP_032492633 YP_009083112
SP P00183
AlphaFold P00183

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks