BMRB Entry 5721

Title:
Backbone resonance assignment of a aminoglycoside-3'-phosphotransferase from Klebsiella pneumoniae
Deposition date:
2003-03-10
Original release date:
2004-02-11
Authors:
Stoldt, Matthias; Kupce, Eriks; Gorlach, Matthias
Citation:

Citation: Stoldt, Matthias; Kupce, Eriks; Rehm, Bernd; Gorlach, Matthias. "Letter to the Editor: Backbone resonance assignment of an aminoglycoside-3'-phosphotransferase type IIa "  J. Biomol. NMR 28, 93-94 (2004).
PubMed: 14739646

Assembly members:

Assembly members:
Aminoglycoside-3'-phosphotransferase, polymer, 264 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Klebsiella pneumoniae   Taxonomy ID: 573   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Klebsiella pneumoniae

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts449
13C chemical shifts709
15N chemical shifts233

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1APH(3')II monomer1

Entities:

Entity 1, APH(3')II monomer 264 residues - Formula weight is not available

1   METILEGLUGLNASPGLYLEUHISALAGLY
2   SERPROALAALATRPVALGLUARGLEUPHE
3   GLYTYRASPTRPALAGLNGLNTHRILEGLY
4   CYSSERASPALAALAVALPHEARGLEUSER
5   ALAGLNGLYARGPROVALLEUPHEVALLYS
6   THRASPLEUSERGLYALALEUASNGLULEU
7   GLNASPGLUALAALAARGLEUSERTRPLEU
8   ALATHRTHRGLYVALPROCYSALAALAVAL
9   LEUASPVALVALTHRGLUALAGLYARGASP
10   TRPLEULEULEUGLYGLUVALPROGLYGLN
11   ASPLEULEUSERSERHISLEUALAPROALA
12   GLULYSVALSERILEMETALAASPALAMET
13   ARGARGLEUHISTHRLEUASPPROALATHR
14   CYSPROPHEASPHISGLNALALYSHISARG
15   ILEGLUARGALAARGTHRARGMETGLUALA
16   GLYLEUVALASPGLNASPASPLEUASPGLU
17   GLUHISGLNGLYLEUALAPROALAGLULEU
18   PHEALAARGLEULYSALAARGMETPROASP
19   GLYGLUASPLEUVALVALTHRHISGLYASP
20   ALACYSLEUPROASNILEMETVALGLUASN
21   GLYARGPHESERGLYPHEILEASPCYSGLY
22   ARGLEUGLYVALALAASPARGTYRGLNASP
23   ILEALALEUALATHRARGASPILEALAGLU
24   GLULEUGLYGLYGLUTRPALAASPARGPHE
25   LEUVALLEUTYRGLYILEALAALAPROASP
26   SERGLNARGILEALAPHETYRARGLEULEU
27   ASPGLUPHEPHE

Samples:

sample_1: Aminoglycoside-3'-phosphotransferase 1.0 mM

cond_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian UnityINOVA 600 MHz
  • Varian UnityINOVA 750 MHz
  • Varian UnityINOVA 900 MHz

Related Database Links:

PDB
DBJ BAA21468 BAA21470 BAA23653 BAA78209 BAA94847
EMBL CAA03938 CAA07593 CAA23892 CAA54105 CAA54106
GB AAA03699 AAA56726 AAA61621 AAA61623 AAA61626
REF WP_000572405 WP_000572406 WP_004614937 WP_017432000 WP_024031215
SP P00552
AlphaFold P00552

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks