BMRB Entry 5701

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5701
MolProbity Validation Chart

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for the catalytic GEF domain of Salmonella SopE2
Deposition date:
2003-02-18
Original release date:
2003-10-17
Authors:
Williams, Christopher
Citation:

Citation: Williams, Christopher; Galyov, Edoudard; Bagby, Stefan. "Letter to the Editor: Assignment of 1H, 13C and 15N Resonances of the Catalytic Domain of Guanine Nucleotide Exchange Factor SopE2 from Salmonella dublin"  J. Biomol. NMR 26, 379-380 (2003).
PubMed: 12815267

Assembly members:

Assembly members:
Salmonella SopE2, polymer, 172 residues, 18398.1 Da.

Natural source:

Natural source:   Common Name: Salmonella   Taxonomy ID: 28901   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Salmonella enterica subsp enterica ser typhimurium

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Salmonella SopE2: GNASEGRAVLTSKTVKDFML QKLNSLDIKGNASKDPAYAR QTCEAILSAVYSNNKDQCCK LLISKGVSITPFLKEIGEAA QNAGLPGEIKNGVFTPGGAG ANPFVVPLIASASIKYPHMF INHNQQVSFKAYAEKIVMKE VTPLFNKGTMPTPQQFQLTI ENIANKYLQNAS

Data sets:
Data typeCount
1H chemical shifts1020
13C chemical shifts680
15N chemical shifts157

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SopE2 monomer1

Entities:

Entity 1, SopE2 monomer 172 residues - 18398.1 Da.

1   GLYASNALASERGLUGLYARGALAVALLEU
2   THRSERLYSTHRVALLYSASPPHEMETLEU
3   GLNLYSLEUASNSERLEUASPILELYSGLY
4   ASNALASERLYSASPPROALATYRALAARG
5   GLNTHRCYSGLUALAILELEUSERALAVAL
6   TYRSERASNASNLYSASPGLNCYSCYSLYS
7   LEULEUILESERLYSGLYVALSERILETHR
8   PROPHELEULYSGLUILEGLYGLUALAALA
9   GLNASNALAGLYLEUPROGLYGLUILELYS
10   ASNGLYVALPHETHRPROGLYGLYALAGLY
11   ALAASNPROPHEVALVALPROLEUILEALA
12   SERALASERILELYSTYRPROHISMETPHE
13   ILEASNHISASNGLNGLNVALSERPHELYS
14   ALATYRALAGLULYSILEVALMETLYSGLU
15   VALTHRPROLEUPHEASNLYSGLYTHRMET
16   PROTHRPROGLNGLNPHEGLNLEUTHRILE
17   GLUASNILEALAASNLYSTYRLEUGLNASN
18   ALASER

Samples:

sample_1: Salmonella SopE2, [U-95% 13C; U-90% 15N], 0.5 – 1.0 mM

Ex-cond_1: pH: 7.0 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-1H NOESYnot availablenot availablenot available
1H-1H TOCSYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCACOnot availablenot availablenot available
HNCOCAnot availablenot availablenot available
HCCONHnot availablenot availablenot available
CCONHnot availablenot availablenot available
HAHBCBCACONNHnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
NH2-SELECTIVE 1H-15N HSQCnot availablenot availablenot available
CN NOESYnot availablenot availablenot available

Software:

SPARKY - peak analysis

NMR spectrometers:

  • VARIAN INOVA 600 MHz

Related Database Links:

PDB
DBJ BAJ36820 BAP07723
EMBL CAR32763 CAR37140 CAR59099 CBG24854 CBW17878
GB AAF63159 AAF91225 AAK27350 AAK27351 AAL20770
REF NP_460811 WP_000182065 WP_000182066 WP_000182067 WP_000182068
SP Q5PHN0 Q7CQD4
AlphaFold Q5PHN0 Q7CQD4 Q5PHN0 Q7CQD4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks