BMRB Entry 5680

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5680

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Title:
Letter to the Editor: Sequence-specific assignment and secondary structure determination of the 195-residue complex formed by the Mycobacterium tuberculosis proteins CFP-10 and ESAT-6. Towards an understanding of their role in tuberculosis pathogenesis
Deposition date:
2003-01-31
Original release date:
2004-11-29
Authors:
Renshaw, Philip; Veverka, Vaclav; Kelly, Geoff; Frenkiel, Thomas; Williamson, Richard; Gordon, Stephen; Hewinson, R.; Carr, Mark
Citation:

Citation: Renshaw, Philip; Veverka, Vaclav; Kelly, Geoff; Frenkiel, Thomas; Williamson, Richard; Gordon, Stephen; Hewinson, R.; Carr, Mark. "Letter to the Editor: Sequence-specific assignment and secondary structure determination of the 195-residue complex formed by the Mycobacterium tuberculosis proteins CFP-10 and ESAT-6. "  J. Biomol. NMR 30, 225-226 (2004).

Assembly members:

Assembly members:
Culture Filtrate Protein of 10 kDa, polymer, 100 residues, 10662 Da.
ESAT-6, polymer, 95 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mycobacterium tuberculosis   Taxonomy ID: 1773   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Mycobacterium tuberculosis and bovis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Culture Filtrate Protein of 10 kDa: MAEMKTDAATLAQEAGNFER ISGDLKTQIDQVESTAGSLQ GQWRGAAGTAAQAAVVRFQE AANKQKQELDEISTNIRQAG VQYSRADEEQQQALSSQMGF
ESAT-6: MTEQQWNFAGIEAAASAIQG NVTSIHSLLDEGKQSLTKLA AAWGGSGSEAYQGVQQKWDA TATELNNALQNLARTISEAG QAMASTEGNVTGMFA

Data sets:
Data typeCount
1H chemical shifts1141
13C chemical shifts533
15N chemical shifts194

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CFP-101
2ESAT-62

Entities:

Entity 1, CFP-10 100 residues - 10662 Da.

1   METALAGLUMETLYSTHRASPALAALATHR
2   LEUALAGLNGLUALAGLYASNPHEGLUARG
3   ILESERGLYASPLEULYSTHRGLNILEASP
4   GLNVALGLUSERTHRALAGLYSERLEUGLN
5   GLYGLNTRPARGGLYALAALAGLYTHRALA
6   ALAGLNALAALAVALVALARGPHEGLNGLU
7   ALAALAASNLYSGLNLYSGLNGLULEUASP
8   GLUILESERTHRASNILEARGGLNALAGLY
9   VALGLNTYRSERARGALAASPGLUGLUGLN
10   GLNGLNALALEUSERSERGLNMETGLYPHE

Entity 2, ESAT-6 95 residues - Formula weight is not available

1   METTHRGLUGLNGLNTRPASNPHEALAGLY
2   ILEGLUALAALAALASERALAILEGLNGLY
3   ASNVALTHRSERILEHISSERLEULEUASP
4   GLUGLYLYSGLNSERLEUTHRLYSLEUALA
5   ALAALATRPGLYGLYSERGLYSERGLUALA
6   TYRGLNGLYVALGLNGLNLYSTRPASPALA
7   THRALATHRGLULEUASNASNALALEUGLN
8   ASNLEUALAARGTHRILESERGLUALAGLY
9   GLNALAMETALASERTHRGLUGLYASNVAL
10   THRGLYMETPHEALA

Samples:

sample_1: Culture Filtrate Protein of 10 kDa, [U-13C; U-15N], 0.9 – 1.5 mM; ESAT-6, [U-13C; U-15N], 0.9 – 1.5 mM

condition_1: pH: 6.5 na; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
TOCSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
15N/1H HSQCnot availablenot availablenot available
15N/1H TOCSY-HSQCnot availablenot availablenot available
15N/1H NOESY-HSQCnot availablenot availablenot available
13C/1H HCCH-TOCSYnot availablenot availablenot available
13C/1H HMQC-NOESYnot availablenot availablenot available
15N/13C/1H HNCACBnot availablenot availablenot available
15N/13C/1H CBCA(CO)NHnot availablenot availablenot available
15N/13C/1H HAHB(CBCACO)NHnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Bruker AVANCE 600 MHz

Related Database Links:

PDB
DBJ BAL68013 BAQ08112 GAA43757
EMBL CAA56099 CAD96091 CCC28958 CCC46228 CCE39298 CAE55648 CAD96091 CAA56099
GB AAC44033 AAC83446 AAK48357 AAL16896 AAO62007
PRF 2209337A 2209337A
REF NP_338543 NP_857542 WP_003399963 WP_003909104 WP_023349865 YP_002077444 YP_001289837 YP_001285265 NP_857542 NP_338543
SP P0A565 P9WNK6 P9WNK7
SWISS-PROT P0A565 P0A564
GenBank AAO62007 AAL16896 AAK48357 AAC83446 AAC44033
AlphaFold P0A565 P9WNK6 P9WNK7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks