BMRB Entry 5601

Click here to enlarge.

PDB ID: 1zrp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5601
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Complete 1H,13C,15N assignments of zinc Pyrococcus furiosus rubredoxin
Deposition date:
2002-11-26
Original release date:
2003-05-14
Authors:
Bougault, Catherine; Eidsness, Marly; Prestegard, James
Citation:

Citation: Bougault, Catherine; Eidsness, Marly; Prestegard, James. "Hydrogen Bonds in Rubredoxins from Mesophilic and Hyperthermophilic Organisms"  Biochemistry 42, 4357-4372 (2003).
PubMed: 12693931

Assembly members:

Assembly members:
pyrococcus furiosus rubredoxin, polymer, 54 residues, 6443.68 Da.
pyrococcus furiosus rubredoxin, polymer, 54 residues, 6414.67 Da.
pyrococcus furiosus rubredoxin, polymer, 53 residues, 6277.52 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Pyrococcus furiosus   Taxonomy ID: 2261   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrococcus furiosus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pEt T7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
pyrococcus furiosus rubredoxin: XAKWVCKICGYIYDEDAGDP DNGISPGTKFEELPDDWVCP ICGAPKSEFEKLED
pyrococcus furiosus rubredoxin: MAKWVCKICGYIYDEDAGDP DNGISPGTKFEELPDDWVCP ICGAPKSEFEKLED
pyrococcus furiosus rubredoxin: AKWVCKICGYIYDEDAGDPD NGISPGTKFEELPDDWVCPI CGAPKSEFEKLED

Data typeCount
1H chemical shifts476
15N chemical shifts159
13C chemical shifts550
coupling constants108

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1fMetPfRdZn1
2Zn14
3MetPfRdZn2
4Zn24
5WT-PfRdZn3
6Zn34

Entities:

Entity 1, fMetPfRdZn 54 residues - 6443.68 Da.

1   FMEALALYSTRPVALCYSLYSILECYSGLY
2   TYRILETYRASPGLUASPALAGLYASPPRO
3   ASPASNGLYILESERPROGLYTHRLYSPHE
4   GLUGLULEUPROASPASPTRPVALCYSPRO
5   ILECYSGLYALAPROLYSSERGLUPHEGLU
6   LYSLEUGLUASP

Entity 4, Zn1 - Zn - 65.409 Da.

1   ZN

Entity 2, MetPfRdZn 54 residues - 6414.67 Da.

1   METALALYSTRPVALCYSLYSILECYSGLY
2   TYRILETYRASPGLUASPALAGLYASPPRO
3   ASPASNGLYILESERPROGLYTHRLYSPHE
4   GLUGLULEUPROASPASPTRPVALCYSPRO
5   ILECYSGLYALAPROLYSSERGLUPHEGLU
6   LYSLEUGLUASP

Entity 3, WT-PfRdZn 53 residues - 6277.52 Da.

1   ALALYSTRPVALCYSLYSILECYSGLYTYR
2   ILETYRASPGLUASPALAGLYASPPROASP
3   ASNGLYILESERPROGLYTHRLYSPHEGLU
4   GLULEUPROASPASPTRPVALCYSPROILE
5   CYSGLYALAPROLYSSERGLUPHEGLULYS
6   LEUGLUASP

Related Database Links:

SWISS-PROT P24297 P24297 P24297
REF NP_579011.1 NP_579011.1 NP_579011.1
GenBank AAL81406.1 AAF03228.1 AAB20233.1 AAL81406.1 AAF03228.1 AAB20233.1 AAL81406.1 AAF03228.1 AAB20233.1
PDB 1BQ9 1BQ8 1ZRP 1VCX 1CAD 1CAA 1BRF
BMRB 5601 5601 1991 5601 5601 1991 5601 5601 1991

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks