BMRB Entry 5378

Name: NMR Structure of a Variant Human Prion Protein with Two Disulfide Bridges
Published: 2003-02-20

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5378

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Structure of a Variant Human Prion Protein with Two Disulfide Bridges

Deposition date:

2002-05-17

Original release date:

2003-02-20

Authors:

Zahn, Ralph; Guntert, Peter; von Schroetter, Christine; Wuthrich, Kurt

Citation:

Citation: Zahn, Ralph; Guntert, Peter; von Schroetter, Christine; Wuthrich, Kurt. "NMR Structure of a Variant Human Prion Protein with Two Disulfide Bridges" J. Mol. Biol. 326, 225-234 (2003).
PubMed: 12547204

Assembly members:

Assembly members:
human prion protein 121-230, polymer, 112 residues, 13092 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human prion protein 121-230: GSVVGGLGGYMLGSAMSRPI IHFGSDYEDRYYRENMHRYP NQVYYRPCDEYSNQNNFVHD CVNITIKQHTVTTTTKGENF TETDVKMMERVVEQMCITQY ERCSQAYYQRGS

Data sets:

assigned_chemical_shifts
- CSS

Data type	Count
1H chemical shifts	770
13C chemical shifts	321
15N chemical shifts	130

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hprp(121-230) subunit 1	1

Entities:

Entity 1, hprp(121-230) subunit 1 112 residues - 13092 Da.

1

GLY

SER

VAL

GLY

LEU

GLY

TYR

2

MET

LEU

GLY

SER

ALA

MET

SER

ARG

PRO

ILE

3

ILE

HIS

PHE

GLY

SER

ASP

TYR

GLU

ASP

ARG

4

TYR

ARG

GLU

ASN

MET

HIS

ARG

TYR

PRO

5

ASN

GLN

VAL

TYR

ARG

PRO

CYS

ASP

GLU

6

TYR

SER

ASN

GLN

ASN

PHE

VAL

HIS

ASP

7

CYS

VAL

ASN

ILE

THR

ILE

LYS

GLN

HIS

THR

8

VAL

THR

LYS

GLY

GLU

ASN

PHE

9

THR

GLU

THR

ASP

VAL

LYS

MET

GLU

ARG

10

VAL

GLU

GLN

MET

CYS

ILE

THR

GLN

TYR

11

GLU

ARG

CYS

SER

GLN

ALA

TYR

GLN

ARG

12

GLY

SER

Samples:

sample_1: human prion protein 121-230, [U-15N; U-13C], 0.7 mM

sample_2: human prion protein 121-230 0.7 mM

Ex-cond_1: pH: 4.5 na; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-1H-15N/13C NOESY	not available	not available	not available
1H-1H-15N TOCSY	not available	not available	not available
HNCA	not available	not available	not available
HSQC	not available	not available	not available

Software:

PROSA -

XEASY -

DYANA-CANDID -

NMR spectrometers:

Bruker DRX 600 MHz
Bruker DRX 750 MHz

BMRB	15676 17756 17780 18426 4379 4402 4434 4620 4736 5713
PDB	1E1G 1E1J 1E1P 1E1S 1E1U 1E1W 1H0L 1HJM 1HJN 1I4M 1QLX 1QLZ 1QM0 1QM1 1QM2 1QM3 2K1D 2LFT 2LSB 2W9E 3HAK 3HER 4DGI 4KML 4N9O
DBJ	BAA00011 BAG32276 BAG32278 BAG32279 BAG52189
EMBL	CAA58442
GB	AAA19664 AAA60182 AAA68633 AAB59442 AAB59443
REF	NP_000302 NP_001073590 NP_001073591 NP_001073592 NP_898902
SP	P04156 P40252
AlphaFold	P04156 P40252