BMRB Entry 53398

Name: Uba7 UFD
Published: 2025-10-21

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PDB ID: 8wwx
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53398
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Uba7 UFD

Deposition date:

2025-10-18

Original release date:

2025-10-21

Authors:

DAG, Cagdas

Citation:

Citation: DAG, Cagdas. "Role of Uba7-UFD in E1-E2 ISGylation: NMR-Based Insights into E2 Binding Dynamics" .

Assembly members:

Assembly members:
entity_1, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: PAGQPERTLESLLAHLQEQH GLRVRILLHGSALLYAAGWS PEKQAQHLPLRVTELVQQLT GQAPAPGQRVLVLELSCEGD DEDTAFPPLHYEL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	249
15N chemical shifts	71
1H chemical shifts	561

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Uba7 UFD	1

Entities:

Entity 1, Uba7 UFD 93 residues - Formula weight is not available

1

PRO

ALA

GLY

GLN

PRO

GLU

ARG

THR

LEU

GLU

2

SER

LEU

ALA

HIS

LEU

GLN

GLU

GLN

HIS

3

GLY

LEU

ARG

VAL

ARG

ILE

LEU

HIS

GLY

4

SER

ALA

LEU

TYR

ALA

GLY

TRP

SER

5

PRO

GLU

LYS

GLN

ALA

GLN

HIS

LEU

PRO

LEU

6

ARG

VAL

THR

GLU

LEU

VAL

GLN

LEU

THR

7

GLY

GLN

ALA

PRO

ALA

PRO

GLY

GLN

ARG

VAL

8

LEU

VAL

LEU

GLU

LEU

SER

CYS

GLU

GLY

ASP

9

ASP

GLU

ASP

THR

ALA

PHE

PRO

LEU

HIS

10

TYR

GLU

LEU

Samples:

sample_1: Uba7 UFD, [U-100% 13C; U-100% 15N], 700 uM; TRIS 20 mM; sodium azide 0.02%; sodium chloride 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.4; pressure: 1 atm; temperature: 20 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz