BMRB Entry 5332

Name: 1H, 13C, and 15N Chemical Shift Assignments for E85Q recoverin
Published: 2002-06-13

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PDB ID: 1jsa
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5332
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N Chemical Shift Assignments for E85Q recoverin

Deposition date:

2002-03-20

Original release date:

2002-06-13

Authors:

Ames, James; Hamasaki, N.; Molchanova, T.

Citation:

Citation: Ames, James; Hamasaki, N.; Molchanova, T.. "Structure and Calcium-binding Studies of a Recoverin Mutant (E85Q) in an Allosteric Intermediate State" Biochemistry 41, 5776-5787 (2002).
PubMed: 11980481

Assembly members:

Assembly members:
Recoverin, polymer, 202 residues, 23333.4 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: Cow Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Recoverin: XGNSKSGALSKEILEELQLN TKFTEEELSSWYQSFLKECP SGRITRQEFQTIYSKFFPEA DPKAYAQHVFRSFDANSDGT LDFKEYVIALHMTSAGKTNQ KLEWAFSLYDVDGNGTISKN EVLEIVTAIFKMISPEDTKH LPEDENTPEKRAEKIWGFFG KKDDDKLTEKEFIEGTLANK EILRLIQFEPQKVKEKLKEK KL

Data sets:

assigned_chemical_shifts
- E85Q_chemical_shifts

Data type	Count
1H chemical shifts	540
13C chemical shifts	523
15N chemical shifts	170

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	recoverin mutant E85Q	1
2	Ca++, I	2
3	Ca++, II	2

Entities:

Entity 1, recoverin mutant E85Q 202 residues - 23333.4 Da.

1

MYR

GLY

ASN

SER

LYS

SER

GLY

ALA

LEU

SER

2

LYS

GLU

ILE

LEU

GLU

LEU

GLN

LEU

ASN

3

THR

LYS

PHE

THR

GLU

LEU

SER

4

TRP

TYR

GLN

SER

PHE

LEU

LYS

GLU

CYS

PRO

5

SER

GLY

ARG

ILE

THR

ARG

GLN

GLU

PHE

GLN

6

THR

ILE

TYR

SER

LYS

PHE

PRO

GLU

ALA

7

ASP

PRO

LYS

ALA

TYR

ALA

GLN

HIS

VAL

PHE

8

ARG

SER

PHE

ASP

ALA

ASN

SER

ASP

GLY

THR

9

LEU

ASP

PHE

LYS

GLU

TYR

VAL

ILE

ALA

LEU

10

HIS

MET

THR

SER

ALA

GLY

LYS

THR

ASN

GLN

11

LYS

LEU

GLU

TRP

ALA

PHE

SER

LEU

TYR

ASP

12

VAL

ASP

GLY

ASN

GLY

THR

ILE

SER

LYS

ASN

13

GLU

VAL

LEU

GLU

ILE

VAL

THR

ALA

ILE

PHE

14

LYS

MET

ILE

SER

PRO

GLU

ASP

THR

LYS

HIS

15

LEU

PRO

GLU

ASP

GLU

ASN

THR

PRO

GLU

LYS

16

ARG

ALA

GLU

LYS

ILE

TRP

GLY

PHE

GLY

17

LYS

ASP

LYS

LEU

THR

GLU

LYS

18

GLU

PHE

ILE

GLU

GLY

THR

LEU

ALA

ASN

LYS

19

GLU

ILE

LEU

ARG

LEU

ILE

GLN

PHE

GLU

PRO

20

GLN

LYS

VAL

LYS

GLU

LYS

LEU

LYS

GLU

LYS

21

LYS

LEU

Entity 2, Ca++, I - Ca - 40.078 Da.

1

CA

Samples:

sample1: Recoverin, [U-13C; U-15N], 0.8 mM

Ex-cond_1: pH: 6.7; temperature: 310 K; ionic strength: 0.05 M

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N NOESY	sample1	not available	Ex-cond_1
1H-15N TOCSY	sample1	not available	Ex-cond_1
HNCACB	sample1	not available	Ex-cond_1
HNCO	sample1	not available	Ex-cond_1
CBCACONH	sample1	not available	Ex-cond_1
HBHACONH	sample1	not available	Ex-cond_1

Software:

No software information available

NMR spectrometers:

Bruker DRX 600 MHz

BMRB	4030
PDB	1IKU 1JSA 1LA3 1OMR 1OMV 1REC 2HET 2I94 4M2O 4M2P 4M2Q 4MLW
EMBL	CAA44928
GB	AAB59256 ELR47741
PRF	1802271A
REF	NP_776590 XP_004012748 XP_005693626 XP_005907603 XP_005959860
SP	P21457
TPG	DAA18803
AlphaFold	P21457 P21457