BMRB Entry 5330
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5330
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Franzoni, Lorella; Luecke, Christian; Perez, Carlos; Cavazzini, Davide; Rademacher, Martin; Ludwig, Christian; Spisni, Alberto; Rossi, Gian Luigi; Rueterjans, Heinz. "Structure and backbone dynamics of apo- and holo-cellular retinol-binding
protein in solution" J. Biol. Chem. 277, 21983-21997 (2002).
PubMed: 11934897
Assembly members:
cellular retinol-binding protein type I, polymer, 135 residues, 15703 Da.
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11b
Entity Sequences (FASTA):
cellular retinol-binding protein type I: MPVDFNGYWKMLSNENFEEY
LRALDVNVALRKIANLLKPD
KEIVQDGDHMIIRTLSTFRN
YIMDFQVGKEFEEDLTGIDD
RKCMTTVSWDGDKLQCVQKG
EKEGRGWTQWIEGDELHLEM
RAEGVTCKQVFKKVH
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
- heteronucl_NOEs
- order_parameters
| Data type | Count |
| T1 relaxation values | 171 |
| T2 relaxation values | 152 |
| order parameters | 89 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cellular retinol-binding protein type I | 1 |
Entities:
Entity 1, cellular retinol-binding protein type I 135 residues - 15703 Da.
| 1 | MET | PRO | VAL | ASP | PHE | ASN | GLY | TYR | TRP | LYS | ||||
| 2 | MET | LEU | SER | ASN | GLU | ASN | PHE | GLU | GLU | TYR | ||||
| 3 | LEU | ARG | ALA | LEU | ASP | VAL | ASN | VAL | ALA | LEU | ||||
| 4 | ARG | LYS | ILE | ALA | ASN | LEU | LEU | LYS | PRO | ASP | ||||
| 5 | LYS | GLU | ILE | VAL | GLN | ASP | GLY | ASP | HIS | MET | ||||
| 6 | ILE | ILE | ARG | THR | LEU | SER | THR | PHE | ARG | ASN | ||||
| 7 | TYR | ILE | MET | ASP | PHE | GLN | VAL | GLY | LYS | GLU | ||||
| 8 | PHE | GLU | GLU | ASP | LEU | THR | GLY | ILE | ASP | ASP | ||||
| 9 | ARG | LYS | CYS | MET | THR | THR | VAL | SER | TRP | ASP | ||||
| 10 | GLY | ASP | LYS | LEU | GLN | CYS | VAL | GLN | LYS | GLY | ||||
| 11 | GLU | LYS | GLU | GLY | ARG | GLY | TRP | THR | GLN | TRP | ||||
| 12 | ILE | GLU | GLY | ASP | GLU | LEU | HIS | LEU | GLU | MET | ||||
| 13 | ARG | ALA | GLU | GLY | VAL | THR | CYS | LYS | GLN | VAL | ||||
| 14 | PHE | LYS | LYS | VAL | HIS |
Samples:
sample_1: cellular retinol-binding protein type I, [U-15N], 1.6 mM
cond_set_1: pH: 6.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N T1 | not available | not available | not available |
| 15N T2 | not available | not available | not available |
| {1H}-15N NOE | not available | not available | not available |
Software:
XWINNMR v1.3 - data acquisition, data processing
RMX vrev. II/1997 - data analysis
MODELFREE v4.01 - data analysis
NMR spectrometers:
- Bruker DMX 500 MHz
- Bruker DMX 600 MHz